한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제27권5호
  • /
  • Pages.370-377
  • /
  • 1999
  • /
  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Xylanase를 생산하는 내열성 Bacillus속 균주의 분리와 효소생산 조건

isolation of Xylanase-producing Thermo-tolerant Bacillus sp. and Its Enzyme Production

  • 박영서 (경원대학교 식품생물공학과) ;
  • 강미영 (경원대학교 식품생물공학과) ;
  • 장학길 (경원대학교 식품생물공학과) ;
  • 박귀근 (경원대학교 식품생물공학과) ;
  • 강종백 (경원대학교 화학과) ;
  • 이정기 (생명공학연구소 미생물효소R.U.) ;
  • 오태광 (생명공학연구소 미생물효소R.U.)
  • 발행 : 1999.10.01
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초록

Thermo-tolerant bacterium producing the xylanase was isolated from soil and identified as Bacillus pumilus. This strain, named Bacillus pumilus TX703, was able to grow ad produce xylanase at the culture temperature of 5$0^{\circ}C$. The maximum xylanase production was obtained when 1%(w/v) birchwood xylan and 1% (w/v) soytone were used as carbon source and nitrogen source, respectively. The biosynthesis of xylanase was under the catabolite repression induced by glucose in the culture medium, and it was completely inhibited in the presence of 0.2% (w/v) glucose. The maximum activity of xylanase was observed from pH8.0 to 9.0 and from 50 to 6$0^{\circ}C$ and the enzyme was highly heat-stable, whose activity remained was over 50% at 8$0^{\circ}C$, and was quite stable from pH5.0 to 10.0.

키워드

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