한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제27권4호
  • /
  • Pages.344-348
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  • 1999
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  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

경쟁적 효소면역 측정법을 이용한 Treponema pallidum 항체 진단시약의 개발 및 평가

Development and Evaluation of a Competitive Enzyme Immunoassay for the Detection of Antibodies to Treponema pallidum

  • 발행 : 1999.08.01
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초록

A competitive enzyme-linked immunosorbent assay(ELISA) for the detection of antibodies to Treponema pallidum(T.pallidum) was developed and evaluated. T.apllidum lysate was immobilized on the surface of microplate wells and horseradish peroxidase labeled human anti-T.pallidum lysate was immobilized on the surface of microplate wells and horseradish peroxidase labeled human anti-T.pallidum was prepared and used as a tracer. The performance of the competitive ELISA was evaluated by using different specimens. The competitive ELISA showed a sensitivity of 100% in a performance panel consisting of serum and plasma with anti-T.pallidum reactivity ranging from negative to strong positive by FTA-ABS test system and 120 plasma samples positive by TPHA. The specificity of the competitive ELISA was 100% in 1,200 plasma samples collected from healthy seronegative blood donors. These results suggest that the competitive ELISA provides an excellent assay method for the detection of antibodies to T.pallidum, and may be particularly useful for serological blood screening of syphilis.

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참고문헌

  1. J. Clin. Microl. v.36 Evaluation of a new competitive immunoassay(bioelisa syphilis) for screening for Treponema pallidum antibodies at various stage of syphilis Anne E.;Loic B.;Jean-Michel A.
  2. Sexually Transmitted Disease v.13 no.4 Four serologic tests for syphilis: Results with comparison of selected groups of sera Carol E.F.;Elizabeth F.H.;Victoria P.;Sandra A.L.;John C.F.
  3. J. Med. Microbiol. v.26 Competitive enzyme-linked immunosorbent assay for Treponema pallidum antibodies CoDD A.A.;Sprott M.S.;Narang H.K.;Crone P.B.;Turner R.H.
  4. Microbiologia v.19 Evaluation of a competitive immunoassay in screening for syphilis De Majo E.;Bianchini G.;Parri F.;Tocci E.;Monaci M.;Paoli C.
  5. J. Immunol. v.97 Protein A from S. aureus. Pseudo-immune reaction with human gammaglobulin Forsgren A.;Sjoquist J.
  6. Nurse Practitioner v.17 Syphilis: Review and Update of the New infection of the 90's Holly B.B.
  7. J. Infect. Dis. v.169 False-positive rapid plasma reagin tests in immunodeficiency virus infection and relationship to anti-cariolipin anti-body and cerum immunoglobulin levels Janice M.R.;Cliff B.;Dave M.;Stephen P.B.
  8. Gemtourin Med. v.68 Laboratory techniques in the diagnosis of syphilis: A review J.J. van der Sluis
  9. Am. J. Obstet. Gynecol. v.163 no.3 False-negative syphilis screening: The prozone phenomenon non immuno hydrops, and diagnosis of syphilis during pregnancy Kathleen B.;Laxmi B.;Harold E.F.
  10. Sexually Transmitted Disease v.11 no.4 Purification of Treponema pallidum, Nichols strain, by percoll density gradient centrifugation Michael A.L.;James N.M.
  11. J .Histochem. Cytochem. v.22 no.12 Peroxidase-labeled antibody. A new method of conjugation Nakane P.K.;Kawaoi A.
  12. J. STD & AIDS v.6 Are you sure it's syphilis? A review of false positive serology Nandwani R.;Evans D.T.P.
  13. Microbiol. Rev. v.57 Treponema pallidum polypeptide research group. Polypeptides of T. pallidum; progress toward under-standing their stuctural, funtional and immunologic roles Norris M.
  14. J. Clin. Microl. v.33 Comparison of CAPTLA syphilis G enzyme immunoassay with rapid plasma reagin test for detection of syphilis Rodger P.S.
  15. Clin. Microbiol. Rev. v.Jan Laboratory diagnosis and interpretation of tests for syphilis Sandra A.J.;Bret M.S.;Andrew H.R.
  16. Sexually Transmitted Disease v.13 no.4 IgG and IgM antibody reactivity to antigens of Treponema pallidum after treatment of syphilis Sharon A.B.;Ronald E.R.;H. Hunter H.;Sheila A. L.
  17. J. Clin. Microbiol. v.15 Evaluation of the microenzyme-linked immunosorbent assay with Treponema pallidum antigen Victoria P.;Elizabeth F.H.;John C.F.
  18. J. Clin. Pathol. v.45 Enzyme immunoassay for anti-treponemal IgG: Screening or confirmatory test? Yong H.;Moyes A.;McMillan A.;Patterson J.
  19. J .STD & AIDS v.3 Syphilis: new diagnostic directions Young H.