Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Advanced Treatment of Swine Wastewater by Botryococcus braunii in a Tubular Bioreactor

Tubular Bioreactor에서 Botryococcus braunii를 이용한 축산폐수의 고도처리

  • 이석준 (생명공학연구소 환경미생물RU) ;
  • 김희식 (생명공학연구소 환경미생물RU) ;
  • 윤병대 (생명공학연구소 환경미생물RU) ;
  • 오희목 (생명공학연구소 환경미생물RU)
  • Published : 1999.04.01
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Abstract

This study was conducted to investigate the production of lipid, and removal of nitrogen and phosphorus from swine wastewater by Botryococcus braunii UTEX 572 in a tubular bioreactor. The rate of dry cell weight increase of B. braunii was highest at 20.1mg/l/din a modified Chu 13 medium at $25^{\circ}C$. Under the above conditions, the rate of lipid content increase was also highest at 6.1mg/l/d. The lipid content of B. braunii on a dry weight basis ranged from 30.5 to 34.1% with an average value of 32.3%. When B. braunii was cultured in a secondary-treated swine wastewater diluted to 50% with tap water, the rate of dry cell weight increase was 18.6mg/l/d and the rate of lipid content increase was 6.0mg/l/d. The lipid content ranged from 30.3 to 34.2%. No significant difference was observed between lipid content and growth conditions. The removal rates of nitrogen and phosphorus in swine wastewater were 43.9% and 41.7%, respectively, after 14 days of incubation.

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References

  1. Appl. Microbiol. Biotechnol. v.29 Photosynthesis, growth and hydrocarbon production of Botryococcus braunii immobilized by entrapment and adsorption in polyurethane foams Bailliez, C.;C. Largeau;E. Casadevall;L. W. Yang;C. Berkaloff
  2. Algal Biotechnology Development of microalgal systems for the production of liquid fuels Barclay, W.;C. Wyman;R. A. Lewin;L. Cheng:Stadler(ed.)
  3. J. Phycol. v.21 Chemical profile of selected species of microalgae with emphasis on lipids Ben-Amotz, A.;T. G. Tornabene;W. H. Thomas
  4. Biotechnol. Bioeng. v.27 Studies on batch and continuous cultures of Botryococcus braunii: Hydrocarbon production in relation to physiological state, cell ultrastructure, and phosphate nutrition Casadevall, E.;D. Dif;C. Largeau;C. Gudin;D. Chaumont;O. Desanti
  5. Limnol. Oceanogr. v.22 Determination of total nitrogen in aqueous samples using persulfate digestion D'Elia, C. F.;P. A. Steudler;N. Corwin
  6. Biological Wastes v.29 Biotreatment of anaerobically digested swine manure with microalgae De la Noue, J.;A. Basseres
  7. Kor. J. Appl. Microbiol. Biotechnol. v.26 Selection of microalgae for advanced treatment of swine wastewater and optimization of treatment condition Kim, S.-B;S. J. Lee;C.-K. Kim;G.-S. Kwon;B.-D. Yoon;H.-M. Oh
  8. FEMS Microbiol. Rev. v.16 Liquid and gaseous fuels from biotechnology: Challenge and opportunities Kosaric, N.;J. Velikonja
  9. Biotechnol. Tech. v.12 Rapid method for the determination of lipids from the green alga Botryococcus braunii Lee, S. J.;B.-D. Yoon;H.-M. Oh
  10. Lett. Appl. Microbiol. v.27 Effects of harvesting time and growth stage on the flocculation of the green alga Botryococcus braunii Lee, S. J.;S.-B. Kim;J.-E. Kim;G.-S. Kwon;B.-D. Yoon;H.-M. Oh
  11. Appl. Biochem. Biotechnol. v.51/52 Carbon dioxide fixation by microalgae photosynthesis using actual fuel gas discharged from a boiler Matsumoto, H.;N. Shioji;A. Hamasaki;Y. Ikuta;Y. Fukuda;M. Sato;N. Endo;T. Tsukamoto
  12. Appl. Biochem. Biotechnol. v.39/40 Stimulation of hydrogen production in algal cells grown under high CO₂concentration and low temperature Miura, Y.;W. Yamada;K. Hirata;K. Miyamoto;M. Kiyohara
  13. Analyt. Chem. Acta v.27 A modified single solution method for the determination of phosphate in natural waters Murphy, J;J. P. Riley
  14. Korean J. Phycol. v.5 Preparation of unialgal cultures from natural waters by a micropipette technique Oh, H.-M.;G.-Y. Rhee
  15. J. Appl. Phycol. v.3 A comparative study of microalgae isolated from flooded rice paddies: Lightlimited growth, C fixation, growth efficiency and relative N and P requirement Oh, H.-M.;G-Y. Rhee
  16. Kor. J. Appl. Microbiol. Biotechnol. v.25 Effects of light intensity and nutrients on the growth of Botryococcus sp. Oh, H.-M.;S.-B. Kim;J.-H. Park;E.-R. Park;S.-T. Lee;G.-S. Kwon;B.-D. Yoon
  17. Kor. J. Limnol. v.31 Deterrnination of limting nutrient for algal growth by algal bioassay Oh, H.-M.;S. J. Lee;S.-B. Kim;M.-K. Park;B.-D. Yoon;D.-H. Kim
  18. Scenedesmus quadricauda. Algae v.13 Advanced treatment of swine wastewater by a green alga Park, M.-K.;S. J. Lee;H.-H. Suh;H.-S. Kim;Y. H. Kim;B.-D. Yoon;H.-M. Oh
  19. Limnol. Oceanogr. v.26 The effect of environmental factors on phytoplankton growth: Light and the interactions of light with nitrate limitation Rhee, G.-Y.;I. J. Gotham
  20. Phytochemistry v.23 Mechanism of non-isoprenoid hydrocarbon biosynthesis in Botryococcus braunii Templier, J.;C. Largeau;E. Casadevall
  21. Phytochemistry v.26 Growth and production of cell constituents in bath cultures of Botryococcus sudeticus Vazquez-Duhalt, R.;H. Greppin
  22. Biotechnol. Bioeng. v.22 Study of a "Bloom" of the oil-rich alga Botryococcus braunii in the Darwin River Reservoir Wake, L. V.;L. W. Hillen
  23. J. Phycol. v.21 Growth and branched hydrocarbon production in a strain of Botryococcus braunii(Chlorophyta) Wolf, F. R.;A. M. Nonomura;J. A. Bassham
  24. Appl. Biochem. Biotech. v.39/40 Biotechnology for production of fuels, chemicals, and materials from biomass Wyman, C. E.;B. J. Goodman
  25. J. Plankton Res. v.8 Effects of light intensity and quality on the relative N and P requirement (the optimum N:P ratio) of marine planktonic algae Wynne, D.;G.-Y. Rhee
  26. Agric. Biol. Chem. v.51 Lipid composition of a green alga, Botryococcus braunii Yamaguchi, K.;H. Nakano;M. Murakami;S. Konosu;O. Nakayama;M. Kanda;A. Nakamura;H. Iwamoto