Physiological Relevance of Salt Environment for in vitro recA System

  • Kim, Jong-Il (Major in Food and Microbial Technology, Faculty of Natural Sciences Seoul Women's University)
  • 발행 : 1999.06.01

초록

RecA protein can promote strand assimilation, homologous pairing, and strand exchange. All these reactions require DNA-dependent ATP hydrolysis by recA protein, and the activities of recA protein are affected by the ionic environment. In this experiment, DNA-dependent ATPase activity showed different sensitivity to anionic species. ATP hydrolysis and strand exchange were relatively sensitive to salt in the reactions with NaCl, strongly inhibited at 100 mM NaCl. However, the inhibition by sodium acetate or sodium glutamate was not observed at 50∼100 mM concentration. Addition of sodium glutamate to the standard reaction condition increased the apparent efficiency of ATP hydrolysis during strand exchange. The condition including 50∼100 mM sodium-glutamate might be similar to the physiological condition.

키워드

참고문헌

  1. J. Biol. Chem. v.259 The mechanism of action of Na-glutamate, lysine-HCl, and piperazine-N₁N¹-bis(2-ethanesulfonic acid) in the stabilization of tubulin and microtubule formation Arakawa, T.;S. N. Timasheff
  2. J. Biol. Chem. v.262 RecA protein-promoted ATP hydrolysis occurs throughout recA nucleoprotein filaments Brenner, S.L.;R.S. Mitchell;S.W. Morrical;S.K. Neuendorf;B.C. Schutte;M.M. Cox
  3. J. Biol. Chem. v.257 RecA protein-promoted DNA strand exchange Cox, M.M.;I.R. Lehman
  4. Ann. Rev. Biochem. v.56 Enzymes of general recombination Cox, M.M.;I.R. Lehman
  5. J. Biol. Chem. v.258 On the role of single-stranded DNA binding protein in recA protein-promoted DNA strand exchange Cox, M.M.;D.A. Soltis;Z. Livneh;I.R. Lehman
  6. J. Mol. Biol. v.157 Characterization of complexes between recA protein and DNA by electronmicroscopy Di Capua, E.;A. Engel;A. Staisiak;T. Koller
  7. J. Mol. Biol. v.200 Structure of helical recA-DNA complex Egelman, E.H.;A. Stasiak
  8. J. Biol. Chem. v.261 On the mechanism of pairing of single-stranded and double-stranded DNA molecules by the recA and single-stranded DNA binding proteins of Escherichia coli Julin, D.A.;P.W. Riddles;I. R. Lehman
  9. J. Biol. Chem. v.261 Topography of the interaction of recA protein with single-stranded deoxyri-bonucleotides Leahy, M.C.;C.M. Radding
  10. Biochemistry v.26 Replacement of potassium chloride by potassium glutamate dramatically enhances protein-DNA interaction in vitro Leirmo, S.;C. Harrison;D.S. Caley;R.R. Burgess;M. T. Record Jr.
  11. J. Mol. Biol. v.187 Salt-dependent changes in the DNA binding cooperativity of Escherichia coli single strand binding protein Lohman, T.M.;L.B. Overman;S. Datta
  12. J. Mol. Biol. v.211 Biochemical properties of the Escherichia coli recA430 protein Menetski, J.P.;S.C. Kowalczykowski
  13. Biochemistry v.25 Continuous association of E. coli single-stranded DNA binding protein with stable complexes of recA protein and single-stranded DNA Morrical, S.W.;J. Lee;M.M. Cox
  14. Microbiol. Rev. v.58 Biochemistry of homologous recombination in Escherichia coli Kowalczykowski, S.C.;D.A. Dixon;A.K. Eggelston;S.C. Lauder;W.M. Rehrauer
  15. J. Biol. Chem. v.263 High salt activation of recA protein ATPase in the absence of DNA Pugh, B.F.;M.M. Cox
  16. J. Biol. Chem. v.262 Variability of the intracellular ionic environment of E. coli differences between in vitro and in vivo effects of ion concentrations on protein-DNA interactions and gene expression Richey, B.;D.S. Caley;M.C Mossing;C. Kolka;C.F. Anderson;T.C. Farrar;M.T. Record Jr.
  17. Molecular Cloning: A Laboratoty Manual(rev. ed.) Sambrook, J.;E.F. Fritsch;T. Maniatis
  18. J. Biol. Chem. v.256 Homologous pairing in genetic recombination Shibata, T R.P. Cunningham;C.M. Radding
  19. J. Biol. Chem. v.258 RecA protein-promoted DNA strand exchange: isolation of recA protein-DNA complexes in the presence of single-stranded DNA binding protein Soltis, D.A.;I.R. Lehman
  20. J. Biol. Chem. v.252 Periplasmic space in Salmonella typhimurium and Escherichia coli Stock, J.B.;B. Rauch;S. Roseman
  21. J. Biol. Chem. v.256 Hydrolysis of nucleoside triphosphates catalyzed by the recA protein of E. coli Weinstock, G.M.;K. McEntee;Lehman, I.R.