YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

In vitro and In vivo Evaluation of Genotoxicity of Stevioside and Steviol, Natural Sweetner

천연감미료 스테비오사이드와 스테비올의 생체내, 시험관내 유전독성평가

  • Published : 1999.10.01
Download PDF
⟨ Previous Next ⟩

Abstract

The standard operation procedure of mouse lymphoma L5178Y $tk^{+/-}-3.7.2C$ gene mutation assay (MOLY) has been regarded as a sensitive in vitro mammalian cell gene mutation assay that is capable of detecting clastogens as well as mutagens. Using MOLY, one of natural sweetner, stevioside (5mg/ml) and its aglycon, steviol ($340{\;}\mu\textrm{g}/ml$) were evaluated the mutagenicity. Stevioside and steviol did not induce mutagenicity in MOLY. On the other hand, stevioside (250mg/kg, B.W.) and steviol (200mg/kg, B.W.) were also evaluated their ability to induce micronuclei in regenerating hepatocytes and bone marrow cells of ddY mice. From these results, stevioside and steviol did not induce any mutagenic effect both MOLY and in vivo micronucleus test.

Keywords

References

  1. Mutat. Res v.16 A mutational assay system using the thymidine kinase locus in mouse lymphoma cells Clive, D.;Flamm, W. G.;Machesko, M. R.;Gernheim,N.J.
  2. Mutat. Res v.111 A comparison of the agar cloning and microtitration techniques for assaying cell curvival and mutation frequency in L5178Y mouse lym-phoma cells Cole, J.;Arlett, C.;Green, M. H. L.;Lowe, J.;Muriel, W.
  3. Vitro Toxicity Testing Protocol v.43 Gene mutation assays in mam-malian cells; Methods in Molecular Biology Julie Clements
  4. Mutat. Res v.113 Revised methods for the Salmonella mutagenicity test Maron, D.M.;Ames, B.N.
  5. Mutat. Res. v.31 The micronucleus test Schmid, W.
  6. Arch. Pathol. v.12 Experimental pathology of the liver.Restoration of liver of the white rat following partial surgical removal Higgins, G. M.;Anderson, R. M.
  7. Mutat. Res. v.334 An improved method of mouse liver micronucleus analysis: an application to age-related genetic alteration and polyploidy study Uryvaeva, I. V.;Delone, G. V.
  8. Environ. Mol. Mutagen v.13 A procedure for data analysis of the rodent micronucleus test involving a historical control Hayashi,M.;Yoshimura, I.;Sofuni,T.;Ishidate,Jr.,M.
  9. Statistical evalution of mutagenicity test data Statistical evaluation of bacteria/mammalian fluctuation test Robinson, W. D.;Healy, M. J. R.;Green, M. H. L.;Cole, J.;Gatehouse, D.;Garner, R. C.;Kirkland D. J.(eds.)
  10. Mutagenesis v.11 Evaluation of the genotoxicity of stevioside and steviol using six in vitro and one in vivo mutagenicity assays Matsui, M.;Matsui, K.;Kawasaki, Y.;Oda, Y.;Noguchi, T.;Kitagawa, Y.;Sawada, M.;Hayashi, M.;Nohmi, T.;Yoshihira, K.;Ishidate, Jr. M.;Sofuni, T
  11. Environ. Health Perspect v.101 Mutagenicity and human chromosomal effect of stevioside, a sweetener from Stevia revaudiana Bertoni Suttajit, M.;Vinitketkaumnuen, U.;Meevatee, U.;Buddhasukh, D
  12. Mutangenesis v.6 Interpertation of results with the 8-azaguanine resistance system in Salnonella typhimurium: no evidence for direct acting mutagenesis Interpretation by 15-oxosteviol, a possoble metabolite of steviol Procinska, E.;Bridges, B. A.;Hanson, R.
  13. Biomed. and Environ. Mass spectrom. v.15 Mass spectral analysis of some derivatives and in vitro metabolites of steviol, the ahlycone of the natural sweeteners, stevioside, erbaudioside A and rubusoside Compadre, D. M.;Hussain, R. A.;Nanayakkara, N. P.;Perzzuto, J. M.;Kinghorn, A. D.
  14. Mutat. Res. v.169 Charac-terization of bacterial mutagenicity mediated 13-hydroxy-ent-kaurenoic acid (stevion) adn several structurally- related derivatives and evalutaion of potential to induce glutathione S-transferase in mice Perzzuto, J. M.;Nanayakkara, N. P.;Compadre, C. M.;Swanson, S. M.;Kinghorn, A. D.;Guenthner, T. M.;Sparnins, V.L.;Lam, L. K.
  15. Proc. Natl. Acad. Sci. USA v.82 Metabolically activated steviol, the aglycone of stevioside, is mutagenic Perzzuto, J. M.;Compadre, C. M.;Swanson, S. M.;Nanayakkara, N. P.;Kinghorn, A. D.
  16. Environ. and Mol. Mutagen v.29 Mouse lymphoma workshop: Victoria, British Columbia, Canada, March 27, 1996 protocol issues regarding the use of the microwell method of the mouse lymphoma assay Sofuni, T.;Wilcox, P.;Shimada, H.;Clements, J.;Honma, M.;Clive, D.;Green, M.;Thybaud, V.;San, R. H. C.;Elliott, B. M.;M ller, L.
  17. Mutat. Res v.369 Re-evaluation of chromosomal aberration induction on nine mouse lymphoma assay 'unique positive' NTP carcinogens Matsuoka, A.;Yamakage, K.;Kusakabe, H.;Wakuri, S.;Asakura, M.;Noguchi, T.;Sugiyama, T.;Shimada, H.;Nakayama, S.;Kasahara, Y.;Takahashi, Y.;Miura, K. F.;Hatanaka, M.;Ishidate, Jr, M.;Morita, T.;Watanabe, K.;Hara, M.;Odawara, K.;Tanaka, N;Hayashi, M.;Sofuni, T
  18. Environ. and Mol. Mutagen. v.25 International workshop on mouse lymphoma assay testing practices and data interpretations: Portland, Oregon, May 7, 1994 Garriott, M. L.;Casciano, D. A.;Schechtman, L. M.;Probst, G. S.
  19. Environ. and Mol. Mutagen. v.25 Consensus agreement regarding protocol issues discussed during the mouse lymphoma workshop:Portland, Oregon, May 7, 1994 Clive, D.;Blocsfoldi, G.;Clements, J.;Cole, J.;Honma, M.;Majeska, J.;Moore, M.;Muller, L.;Myhr, B.;Oberly, T.;Oudelhkim, M.;Rudd, C.;Shimada, H.;Sofuni, T.;Thybaud, V.;Wilcox, P
  20. Mutat. Res. v.189 Guide for performing the mouse lymphoma assay for mam-malian cell mutagenicity Clive,D.;Caspary,W.;Kirby,P.E.;Krehl,R.;Moore,M.;Mayo,J.;Oberly,T.J.
  21. Mutat. Res. v.31 Laboratory proce-dure for assessing specific locus mutations at the tk locus in cultured L5178Y mouse lymphoma cells Clive, D.;Spector, J.F.S.
  22. Mutat. Res v.15 Analasis if TFTr mutants of L5178Y $TK^+/-$ mouse lymphoma cells Moore,M.M.;Clive,D.;Hozier,J.C.;Howard,B.E.;Batson,A.G.;Tuner,N.T.;Sawyer,J.
  23. Environ. and Mol. Mutagen. v.29 An evaluation of micronucleus induction in bone marrow and in hepatocytes isolated from collagenase perfused liver or from formalin-fixed liver using four-week-old rats treated with known clastogens Parton, J. W.;Garriott, M. L.