Archives of Pharmacal Research

The Pharmaceutical Society of Korea (대한약학회)
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Release of Renal Dipeptidase from Rabbit Renal Proximal Tubules and Its Inhibition by Gentamicin

  • Kang, Bok-Yun (Department of Pharmacy, College of Pharmacy, Chonnam National University) ;
  • We, Jeoung-Soon (Department of Pharmacy, College of Pharmacy, Chonnam National University) ;
  • Choi, Kyong (Department of Biology, College of Natural Science Chonnam National University) ;
  • Lee, Hwanghee-Blaise (Department of Biology, College of Natural Science Chonnam National University) ;
  • Han, Ho-Jae (Department of Veterinary Physiology, College of Veterinary Medicine, Chonnam National University) ;
  • Park, Haeng-Soon (Department of Pharmacy, College of Pharmacy, Chonnam National University)
  • Published : 1999.08.01
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Abstract

Effects of several durgs on rabbit renal proximal tubules were examined for the applicability of renal dipeptidase (RDPase, EC 3. 4. 13. 11) release as a model system to study nephrotoxicity. The proximal tubule prepared by the method of Taub (1990) released RDPase spontaneously in the control experiment which was confirmed by Western blotting. RDPase was also released form cisplatin, lipopolysaccardie (LPS), and indomethacin-treated tubules. Gentamicin inhibited RDPase release in a concentration-dependent manner. This RDPase release system may not be a general model to screen nephrotoxicity but could be a useful source of RDPase purification in a simple and inexpensive way.

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