Journal of fish pathology (한국어병학회지)
- Volume 12 Issue 1
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- Pages.24-31
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- 1999
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- 1226-0819(pISSN)
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- 2233-5412(eISSN)
Qualitative Enzyme-Linked Immunosorbent Assay (ELISA) for the Diagnosis of Edwardsiellosis
Edwardsiellosis의 진단을 위한 정성적 ELISA법
- Kim, Myoung-Sug (Department of Aquatic Life Medicine, College of Fisheries Sciences, Pukyong National University) ;
- Hwang, Eun-A (Department of Aquatic Life Medicine, College of Fisheries Sciences, Pukyong National University) ;
- Huh, Min-Do (Department of Aquatic Life Medicine, College of Fisheries Sciences, Pukyong National University) ;
- Jeong, Hyun-Do (Department of Aquatic Life Medicine, College of Fisheries Sciences, Pukyong National University)
- 김명석 (부경대학교 수산과학대학 수산생명의학과) ;
- 황은아 (부경대학교 수산과학대학 수산생명의학과) ;
- 허민도 (부경대학교 수산과학대학 수산생명의학과) ;
- 정현도 (부경대학교 수산과학대학 수산생명의학과)
- Published : 1999.05.30
Abstract
Optimization and standardization of solid phase enzyme immunoassay were done for the diagnosis of edwardsiellosis in fish. The analyzed degree of immobilized antibody on surface of solid phase with peroxidase saturation method showed the optimized result by using partially purified
어류의 edwardsiellosis를 진단하기 위한 solid phase ELISA법에 대한 최적화 분석을 실시하였다. 부분 정제한 E. tarda Edk-2에 대한 토끼 항혈청을 sodium bicarbonate 완충용액에
Keywords
- Solid phase ELISA;
- Edwardsiellosis;
- Diagnosis;
- Optimization;
- EDTA extracted antigen;
- Heat extracted antigen