Glucose Oxidase에 의(依)하여 손상(損傷)된 배양척수감각신경절세포(培養脊髓感覺神經節細胞)에 대(對)한 음양곽(淫羊藿)의 효과(效果)

Effect of Epimedium Koreanum Nakai on GO-Induced Neurotoxicity in Cultured Mouse Spinal Dorsal Root Ganglion Neurons

  • 박승택 (원광대학교 의약자원 연구센터,원광대학교 의과대학 해부학교실) ;
  • 이호섭 (원광대학교 한의과대학 생리학교실) ;
  • 윤용갑 (원광대학교 한의과대학 방제학교실) ;
  • 박병림 (원광대학교 의과대학 생리학교실)
  • Park Seung-Taeck (Medicinal Resources Research Center of Wonkwang University,Department of Anatomy, School of Medicine, Wonkwang University) ;
  • Lee Ho-Sub (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Yun Yong-Gap (Department of Prescription, College of Oriental Medicine, Wonkwang University) ;
  • Park Byung-Rim (Department of Physiology, School of Medicine, Wonkwang University)
  • 발행 : 1999.11.25

초록

척수감각신경절세포에 대한 산소자유기의 신경독성효과에 대한 기전을 규명하기 위하여 여러 농도의 Glucose Oxidase(GO)를 배양 척수 감각신경절세포에 처리한 후 GO의 독성효과를 분석하였으며 또한 GO에 의하여 유발된 신경독성에 대한 음양곽(Epimedium Koreanum Nakai)의 방어효과를 MTT assay법에 의하여 조사하여 다음과 같은 결론을 얻었다. GO는 신경세포에 처리한 농도와 시간에 비례하여 세포의 생존율을 유의하게 감소시켰으며, 또한 음양곽이 GO의 독성효과를 효과적으로 방어하였다. 이상의 결과로부터 산소자유기인 GO는 생쥐의 배양 척수감각신경절세포에 독성을 나타냈으며 음양곽과 같은 한약추출물이 GO의 독성을 방어하는데 효과적인 것으로 나타났다.

To evaluate the neurotoxic effect of oxygen radicals in cultured mouse spinal dorsal root ganglion(DRG) neurons, cytotoxicity was determined by MTT assay after cultured DRG neurons were grown in the media containing various concentrations of glucose oxidase(GO). In addition, neuroprotective effect of herb extract, Epimedium Koreanum Nakai was examined by MTT assay in cultured DRG neurons. Cell viability of cultured DRG neurons was remarkably decreased by GO in dose- and time-dependent manner, and Epimedium Koreanum Nakai protected remarkably GO-induced neurotoxicity in these cultures. From the above results, it is suggested that oxygen radicals is toxic in cultured mouse DRG neurons, and herb extracts such as Epimedium Koreanum Nakai are effective in prevention of the neurotoxicity induced by oxygen radicals in cultured mouse DRG neurons.

키워드

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