Korean Journal of Food Science and Technology (한국식품과학회지)
- Volume 31 Issue 3
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- Pages.593-599
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- 1999
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- 0367-6293(pISSN)
Measurement of Biogenic Amines with a Chitopearl Enzyme Reactor
Chitopearl 효소 Reactor를 이용한 Biogenic Amines 측정
- Park, In-Seon (Korea Food Research Institute) ;
- Kim, Dong-Kyung (Korea Food Research Institute) ;
- Shon, Dong-Hwa (Korea Food Research Institute) ;
- Cho, Yong-Jin (Korea Food Research Institute) ;
- Kim, Nam-Soo (Korea Food Research Institute)
- Published : 1999.06.30
Abstract
Substrate specificity of a flow-injection-analysis (FIA)-type biogenic amine sensor with enzyme reactor was determined. The enzyme reactor was prepared with a diamine oxidase immobilized on preactivated chitosan porous beads (Chitopearl) by intramolecular cross-linking via glutaraldehyde. The sensor showed a rapid response to putrescine and a quasi-linear calibration curve was obtained up to 15.0 mM. The optimal pH and temperature of the enzyme reactor system were 7.5 and
Biogenic amine류는 어육의 부패과정 중 생성되는 성분으로 이를 간편하게 측정하기 위하여 diamine oxidase를 amino기가 관능기인 다공질의 Chitopearl beads에 고정화한 후 이를 충진한 효소 reactor를 제조하였다. 고정화 효소 reactor를 이용한 biogenic amine 연속계측 시스템을 사용하여 putrescine 기질에 대해 15.0 mM까지 검량곡선을 얻을 수 있었다. 이 때, biogenic amine 센서의 최대 감응도는