한국균학회지 (The Korean Journal of Mycology)

한국균학회 (The Korean Society of Mycology)
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먹물버섯속(Coprinus)과 눈물버섯속(Psathyrella)의 ITS 영역 염기서열에 의한 계통학적 유연관계 분석

Phylogenetic Relationships of Genera Coprinus and Psathyrella on the Basis of ITS Region Sequences

  • 박동석 (농업과학기술원 분자유전과) ;
  • 고승주 (농업과학기술원 분자유전과) ;
  • 김양섭 (농업과학기술원 분자유전과) ;
  • 석순자 (농업과학기술원 분자유전과) ;
  • 류진창 (농업과학기술원 분자유전과) ;
  • 성재모 (강원대학교 농생물학과)
  • Park, Dong-Suk (Division of Molecular Genetics, National Institute of Agricultural Science and Technology) ;
  • Go, Seung-Joo (Division of Molecular Genetics, National Institute of Agricultural Science and Technology) ;
  • Kim, Yang-Sup (Division of Molecular Genetics, National Institute of Agricultural Science and Technology) ;
  • Seok, Soon-Ja (Division of Molecular Genetics, National Institute of Agricultural Science and Technology) ;
  • Ryu, Jin-Chang (Division of Molecular Genetics, National Institute of Agricultural Science and Technology) ;
  • Sung, Jae-Mo (Department of Agricultural Biology, Kangwon National University)
  • 발행 : 1999.08.30
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초록

먹물버섯속 및 눈물버섯속 균류의 계통학적 유연관계 분석을 위해 rDNA의 cluster 중 ITS 영역을 Polymerase Chain Reaction(PCR)로 증폭하여 염기서열을 밝혔다. 이들의 ITS I, II 영역은 각각 $258{\sim}301\;bp,\;253{\sim}275\;bp$의 염기쌍으로 이루어져 있었으며 균주간 ITS 염기서열의 상동성은 $43.9{\sim}96%$로 나타났다. 이들의 염기서열을 이용해 분류도(tree)를 작성한 결과, Singer의 형태적 분류체계와 거의 유사한 결과를 보였으나 눈물버섯속 균류들은 먹물버섯속 내 그룹 II의 종들과 함께 분지를 형성하였고 먹물버섯속의 의기준종(type species)인 C. comatus는 조사된 다른 동일 속의 종들과의 유사도에서 50%수준의 저조한 상동성을 보여주었다. 이와 같은 결과로 볼 때 먹물버섯류가 기존의 다수 학자들이 보고한 단일 계통진화(monophyletic evolution)를 해온 속이란 점에서 의문점을 남기었고 좀더 많은 조사가 필요하겠으나 눈물버섯속 균류도 먹물버섯속 균류와 분리시키는 것보다는 동일 속으로 분류하는 것이 바람직 할 것으로 사료되었다.

The internal transcribed spacer regions(ITS) of the ribosomal DNA gene repeat from Coprinus and Psathyrella spp. were amplified using polymerase chain reaction (PCR) and sequenced. Sequences from 11 species including Coprinus comatus, C. atramentarius, C. micaceus, C. cinereus, C. rhizophorus, C. radians, C. echinosporus, C. disseminatus, Psathyrella candolleana, P. spadiceogrisea and Stropharia rugosoannulata were compared. The spacer region I and II were $258{\sim}301\;bp\;and\;253{\sim}275\;bp$ in length respectively and partially contained 17S, 5.8S and 25S. The reciprocal homologies of ITS sequences among these strains were in the range of $43.9{\sim}96.0%$. According to the analysis of ITS sequences, Coprinus and Psathyrella spp. were classified into three clusters. Cluster I consisted of Coprinus lagopus, C. cinereus, C. echinosporus, C. rhizophorus, and C. atramentarius. Cluster II comprised C. micaceus, C. radians, C. disseminatus, Psathyrella candolleana, and P. spadiceogrisea. On the other hand C. comatus is in Cluster III with Stropharia rugosoannulata even though this species is belonging to the section Coprinus in morphological aspect. These results suggest that taxonomic position of Psathyrella would better be inculded in genus Coprinus. Coprinus comatus, the type species of Coprinus, gives a doubt on monophyletic evolution and is assumed to be paraphyletic or polyphyletic.

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