The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
권호 표지

Genetic Relationships of Coprinus spp. on the Basis of Sequences in ITS II Region

먹물버섯류(Coprinus spp.)의 ITS II 영역 염기서열에 의한 유연관계 분석

  • Park, Dong-Suk (Division of Molecular Genetics, National Institute of Agricultural Science and Technology) ;
  • Go, Seung-Joo (Division of Molecular Genetics, National Institute of Agricultural Science and Technology) ;
  • Kim, Yang-Sup (Division of Molecular Genetics, National Institute of Agricultural Science and Technology) ;
  • Seok, Soon-Ja (Division of Molecular Genetics, National Institute of Agricultural Science and Technology) ;
  • Song, Jae-Kyeong (Division of Molecular Genetics, National Institute of Agricultural Science and Technology) ;
  • Yeo, Yun-Soo (Division of Molecular Genetics, National Institute of Agricultural Science and Technology) ;
  • Ryu, Jin-Chang (Division of Molecular Genetics, National Institute of Agricultural Science and Technology) ;
  • Sung, Jae-Mo (Department of Agricultural Biology, Kangwon National University)
  • 박동석 (농업과학기술원 분자유전과) ;
  • 고승주 (농업과학기술원 분자유전과) ;
  • 김양섭 (농업과학기술원 분자유전과) ;
  • 석순자 (농업과학기술원 분자유전과) ;
  • 송재경 (농업과학기술원 분자유전과) ;
  • 여윤수 (농업과학기술원 분자유전과) ;
  • 류진창 (농업과학기술원 분자유전과) ;
  • 성재모 (강원대학교 농생물학과)
  • Published : 1999.02.28
Download PDF
⟨ Previous Next ⟩

Abstract

The internal transcribed spacer II regions (ITS II) of the ribosomal DNA gene repeat from Coprinus spp. were amplified using polymerase chain reaction (PCR) and sequenced. Sequences from 11 species including Coprinus comatus, C. atramentarius, C. micaceus, C. lagopus, C. cinereus, C. rhizophorus, C. flocculosus, C. radians, and C. echinosporus were compared. The spacer regions of them were $253{\sim}275$ nucleotide in length and partially contained 5.8S and 25S. The reciprocal homologies of each ITS II sequence among these strains were in the range of $50.6{\sim}100%$. According to the analysis of ITS II sequences, Coprinus spp. were classified into three clusters. Cluster I consisted of Coprinus lagopus, C. cinereus, C. echinosporus, C. rhizophorus, C. niveus, and C. atramentarius. Cluster II comprised C. micaceus, C. flocculosus, C. radians, and C. disseminatus. On the other hand C. comatus is in Cluster III even though this species is belonging to the section Coprinus in morphological aspect. These results suggest that Coprinus comatus, which was considered as a type species of the genus Coprinus in morphological classification, gives a doubt of monophyletic evolution and is assumed to be paraphyletic or polyphyletic.

먹물버섯속(Coprinus spp.)의 분자 생물학적 분류 가능성을 모색하기 위해 rDNA의 cluster중 ITS II영역을 PCR 증폭하여 염기서열을 밝혔다. 이들의 ITS II 영역은 $253{\sim}275$ 염기쌍으로 이루어져 있었으며 균주간 ITS II염기서열의 유사도는 $50.3{\sim}100%$로 나타났다. 이들의 염기서열을 이용해 분류도를 작성한 결과, Singer의 형태적 분류체계와 거의 유사한 결과를 보였으나 이들 그룹의 기준종(type species)인 C. comatus는 기타 종들과의 유사도에서 50% 수준의 저조한 상동성을 보여 주었다. 이와 같은 결과로 볼 때 이 종은 기존의 다수 학자들이 보고한 단일 계통진화를 해온 속이란 점에서는 다소 의문점을 남기었고, ITS II 영역은 종내 변이는 없는 것으로 보여지며 종내의 균주간의 변이가 밝혀진 그룹에 대해서는 종에 대한 재검토가 필요하다고 본다.

Keywords

References

  1. 鮮滿實用林業便覽 鏑木德二
  2. Researches on Fungi, Vol. VII Buller, A.J.H.
  3. Researches on Fungi, Vol. VIII Buller, A.J.H.
  4. Researches on Fungi, Vol. IV Buller, A.J.H.
  5. Systema Mycologicum II Fries, E.M.
  6. Biotechniques v.16 Amplified method for the preparation of fungal genomic DNA for PCR and RAPD analysis Graham, G.C.;Mayer, S.P.;Henry, R.J.
  7. Mycologia v.86 no.1 Phylogenetic relationships among coprinoid taxa and allies based on data from restriction site mapping of nuclear rDNA Hopple, J.S.;Vigalys, R.
  8. Evolution of protein molecules;Mammalian Protein Metabolism Jukes, T.H.;Cantor, C.R.;Munro, H.N.(ed.)
  9. Le Botaniste v.20 Le development et la position taxonomique de l'Agaricus disseminatus Pers Kuhner, R.
  10. Flore Analytique des Champignons Superieurs Kuhner, R.;Romagnesi.
  11. Genetics v.128 Molecular analysis of Coprinus cinereus mating type A factor demonstrates an unexpectedly complex structure May, G.;Le Chevanton, L.;Pukkila, P.J.
  12. Mycologist v.9 no.2 Sequence or Structure?/ A short review on the application of nucleic acid sequence information to fungal taxonomy Mitchell, J.I.;Robert, P.J.;Moss, S.T.
  13. MEGA: Molecular Evolutionary Genetics Analysis Kumar, S.;Tamura, K.;Nei, M.
  14. Symp. Soc. Exp. Biol. v.38 Analysis of meiotic development in Coprinus cinereus;Controlling Events in Meiosis Pukkila, P.J.;Yashar, B.M.;Binninger, D.M.
  15. Persoonia v.10 Developmental anatomy of Coprinus Reijnders, A.F.M.
  16. The Agaricales in Modern Taxonomy Singer, R.
  17. Mol. Biol. Evol. v.4 The neighbor-joining method: a new method for reconstructing phylogenetic trees Saitou, N.;Nei, M.
  18. The origin and evolution of Agaricales;Evolution ine the Higher Basidiomycetes Smith, A.H.;Peterson, R.H.(ed.)
  19. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics;PCR Protocols: A Guide to Methods and Applications White, T.J.;Lee, Burns S.;Taylor, J.;Innis, M.A.(ed.);Gelfand, D.H.(ed.);Sninsky, J.J.(ed.);White, T.J.(ed.)