Korean Journal of Weed Science (한국잡초학회지)

The Korean Society of Weed Science & The Turfgrass Society of Korea Archive (한국잡초학회 & 한국잔디학회)
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Analysis of Genetic Diversity in Echinochloa Species Using Random Amplified Polymorphic DNAs(RAPDs) Markers

RAPD Marker를 이용한 피 수집종의 유연관계 분석

  • Kim, Kil-Ung (Dept. of Agronomy, College of Agriculture, Kyungpook National University) ;
  • Sohn, Jae-Keun (Dept. of Agronomy, College of Agriculture, Kyungpook National University) ;
  • Shin, Dong-Hyun (Dept. of Agronomy, College of Agriculture, Kyungpook National University) ;
  • Kim, Kyung-Min (Dept. of Agronomy, College of Agriculture, Kyungpook National University) ;
  • Kim, Hak-Yoon (Dept. of Agronomy, College of Agriculture, Kyungpook National University) ;
  • Lee, In-Jung (Dept. of Agronomy, College of Agriculture, Kyungpook National University)
  • 김길웅 (경북대학교 농과대학 농학과) ;
  • 손재근 (경북대학교 농과대학 농학과) ;
  • 신동현 (경북대학교 농과대학 농학과) ;
  • 김경민 (경북대학교 농과대학 농학과) ;
  • 김학윤 (경북대학교 농과대학 농학과) ;
  • 이인중 (경북대학교 농과대학 농학과)
  • Received : 1998.02.12
  • Published : 1998.03.30
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Abstract

Echinochloa species maintained by selling for more than 10 years were classified using random amplified polymorphic DNAs(RAPDs) analysis. Seventy-four decamer of randomly sequence markers were used to classify intraspecific variation irt Echinochloa species. The number of amplification products increased with increasing GC content of the primer in the range between 60% and 70% GC. Single-base substitutions of a primer altered amplification, providing new polymorphisms. The size of amplified DNA was mostly between 0.40kbp and 1.4kbp with the most common bands at 1.1kbp. Echinochloa species were detected with 6 primers which generated 26 polymorphic amplified DNAs. By hierarchical cluster analysis, Echinochloa species collected in Korea were divided into three groups. These results revealed that RAPD markers are useful tools for the determination of genetic variations in Echinochloa species.

피속 잡초 수집종 33종을 대상으로 RAPD marker를 이용하여 피 수집종 간의 유전적변이를 알아보고, 수집종들을 판별할 수 있는 DNA marker를 선발하기 위하여 본 실험을 수행한 결과를 요약하면 다음과 같다. Operon사에서 제작된 74개의 10-mer RAPD primer 가운데에서 명확한 다형성을 보이는 6개 primer를 선발하였다. 이들 primer로 PCR에서 증폭된 밴드는 31개이었으며 이 가운데 다형성을 나타내는 band는 26개(83.9%)로 나타났다. 피 수집종 간의 유연 관계를 분석한 결과 공시된 피 수집종은 크게 3그룹으로 분류할 수 있었다.

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