Journal of Korean Ophthalmic Optics Society (한국안광학회지)

The Korean Ophthalmic Optics Society (한국안광학회)
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Development of Visual System in Korean Salamander(Hynobius leechi)

도룡뇽(Hynobius leechi)의 시각계 발생

  • Cheong, Seon-Woo (Department of Biology, College of Natural Sciences, Changwon National University) ;
  • Yoon, Chun-Sik (Department of Molecular and Cell Biology, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Douk-Hoon (Department of Ocular Optics, College of Masan)
  • 정선우 (창원대학교 자연과학대학 생물학과) ;
  • 윤춘식 (생명공학연구소 분자세포생물학연구부) ;
  • 김덕훈 (마산대학 안경광학과)
  • Received : 1988.11.24
  • Published : 1998.12.31
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Abstract

Visual system of Korean Salamander(Hynobius leechi) was morphologically studied. Fertilized eggs in egg sacs were collected and were developed in sterile saline solution. Various sized larvae of 5-30mm in length were fixed. Specimens were paraffin sectioned and were observed under light microscope. In 5mm length larva, lens rudiment induced by optic cup was combined with sensory ectoderm. The shape of lens was changed as spherical in 12mm length larva, but the retinal layer did not differentiated into three layers. The differentiation of retinal layer was clear in 14-16mm length larva. The central region of lens fibers was degenerated. Iris and ciliary body were formed from the marginal zone of optic cup in 20mm length larva. Choroid was thicker in elder eye of 30mm length larva and cartilage developed at outer region of optic cup. The outer segment of photoreceptor cell layer grew longitudinally. Optic nerve was connected to the ventral part of brain through cartilage.

한국산 도룡농 Hynobius leechi의 시각계발생을 형태학적으로 연구하였다. 수정란을 채집하여 멸균된 생리식염수에서 발생시키고 5-30mm길이의 유생을 크기별로 하였다. 표본은 paraffin에 포매하고 절편하여 광학현미경하에서 관찰하였다. 5mm유생에서, 안배에 의해 유도된 수정체의 원기는 감각외 배엽과 결함되어 있었다. 12mm유생은 수정체가 구형으로 되고 색소상피가 뚜렷해지나 망막층의 분화는 일어나지 않았다. 14-16mm유생은 망막층의 분화가 뚜렷해지며 수정체의 중심부섬유의 핵이 붕괴되었다. 20mm유생의 안배의 외연부는 얇아져서 홍채와 모양체를 형성하였다. 30mm유생에서는 맥락막이 두터워지고 연골이 안구의 외곽에서도 나타났다. 광수용세포층의 외절은 점차로 길게 성장하고 안구신경은 연골을 지나 뇌의 복측에 연결된다.

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