YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Screening of In vitro DNA-binding Activity from the Crude Drugs

생약으로부터 In vitro DNA 결합활성의 검색

  • Published : 1998.04.01
Download PDF
⟨ Previous Next ⟩

Abstract

One hundred and seventeen crude drugs were screened for DNA-binding activity in vitro. The DNA-methyl green assay is a useful biochemical screen for the detection or development of biologically active compounds which bind to DNA. This assay is based upon the fact that free methyl green undergoes rapid spontaneous molecular rearrangement to its colorless carbinol base so that the liberation of the dye from DNA by displacement can be followed spectrophotometrically as a decrease in absorbance at 65Onm. Seven methanolic extracts of crude drugs including Cinnamomi Cortex spissus, Coicis Semen, Coptidis Rhizoma, Perillae Semen, Plantaginis Semen, Polygalae Radix and Zanthoxyli Fructus showed less than 2,000${\mu}$g/ml as their $IC_{50}$ values. The active principles of Plantaginis Semen and Zanthoxyli Fructus were transferred into organic solvents, which showed the $IC_{50}$ values with 588 and 574${\mu}$g/ml, respectively. These fractions have been selected for isolation of biologically active constituents.

Keywords

References

  1. J. Lab. & Clin. Med. v.60 Reaction between DNA and quinacrine and other antimalarials Kurnick, N.B.;Radcliffe, I.E.
  2. Cancer Res. v.29 Studies on the mechanism of action of anthramycin methyl ether, a new antitumor antibiotic Bates, H.M.;Kuenzig, W.;Watson, W.B.
  3. Experientia v.27 On the immunodepressive action of adriamycin Isetta, A.M.;Intini, C.;Soldati, M.
  4. Biochemistry v.14 Studies on the methyl green-DNA complex and its dissociation by drugs Krey, A.K.;Hahn, F.E.
  5. Arch. Biochem. v.29 The determination of deoxyribonuclease activity by methyl green, application to serum Kurnick, N.B.
  6. J. Gen. Physiol. v.34 Methyl green Ⅲ. Reaction with desoxyribonucleic acid, stoichiometry, behavior of the reaction product Kurnick, N.B.;Foster, M.W.
  7. J. Nat. Prod. v.55 A colorimetric microassay for the detection of agents that interact with DNA Burres, N.S.;Frigo, A.;Rasmussen, R.R.;McAlpine, J.B.
  8. J. Ethnopharmacol. v.50 Studies on the cytotoxicity, antimicrobial and DNA-binding activeties of plants used by the Ese'ejas Desmarchelier, C.;Mongelli, E.;Coussio, J.;Ciccia, G.