YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Purification of Anti-allergic Compound from Mori Cortex Radicis Extract

상백피 추출물 중의 항알러지 활성 성분의 분리

  • Lee, Eun-Ju (College of Pharmacy, Seoul National University) ;
  • Chae, Ok-Hee (School of Medicine, Chonbuk National University) ;
  • Lee, Moo-Sam (School of Medicine, Chonbuk National University) ;
  • Lee, Hern-Ku (School of Medicine, Chonbuk National University) ;
  • Huh, Hoon (College of Pharmacy, Seoul National University)
  • Published : 1998.08.01
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Abstract

Mori Cortex Radicis, the root bark of mulberry tree, has been used in the treatment of bronchial asthma and other lung diseases in traditional medicine. There was a recent repor t that the water soluble part with molecular weight of above 10,000 has anti-allergic activity. Therefore, we intended to isolate and purify the anti-allergic compound from hot water extract of the Mori Cortex Radicis. Crude extract of Mori Cortex Radicis was prepared by hot-water extraction, and anti-allergic compound was further purified by alcohol precipitation, successive ultrafiltration, anion exchange chromatography and gel filtration chromatography. This compound had homogeneity which was shown by the sharp single peak in HPLC chromatogram (TSK-GEL G400OPW column, RI detector). The molecular weight of the compound was estimated as 23Kda on the basis of calibration curve plotted against protein standards. This compound was identified as complex of sugar, protein and lignin (19.2: 5.9: 72.7), and proteolysis could not decrease the anti-allergic activity but mild delignification decreased the activity remarkably. Therefore, we concluded that the anti-allergic compound of Mori Cortex Radicis was a lignin-carbohydrate complex.

Keywords

References

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