Journal of Microbiology and Biotechnology
- Volume 8 Issue 6
- /
- Pages.719-722
- /
- 1998
- /
- 1017-7825(pISSN)
- /
- 1738-8872(eISSN)
Could Organic Solvents Be Used for the Alteration of Flux of Hydrophobic Intermediates through a Metabolic Pathway in Microorganisms\ulcorner
- Zucchi, Gioia (Department of Macromolecular Sciences, Institute of Food Research) ;
- Khan, Jeffrey-A. (Department of Macromolecular Sciences, Institute of Food Research) ;
- Vulfson, Evgeny-N. (Department of Macromolecular Sciences, Institute of Food Research)
- Published : 1998.12.01
Abstract
The addition of decane to biotransfonnation media containing Yarrowia lipolytica led to the accumulation of intennediate L-phenylacetaldehyde and L-phenethyl acetate during bioconversion of L-phenylalanine, whilst none of these products were obtained in conventional aqueous fennentations. The results obtained support an earlier hypothesis (Spinnler et al. 1996. Proc. Natl. A cad. Sci. USA 93: 3373-3376) that organic solvents, acting as "thermodynamic traps" for hydrophobic intermediates, can substantially alter metabolic fluxes.
Keywords
- Microorganism;
- metabolic pathways;
- hydrophobic intennediates;
- organic solvents;
- phenethyl alcohol;
- phenethyl acetate;
- phenylacetaldehyde;
- yeast;
- solvent engineering