Journal of Microbiology and Biotechnology
- Volume 8 Issue 3
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- Pages.284-286
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- 1998
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- 1017-7825(pISSN)
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- 1738-8872(eISSN)
Molecular Cloning and Sequence of URA5 Gene Encoding Orotate Phosphoribosyl Transferase (OPRTase) from Entomopathogenic Fungus, Metarhizium anisopliae
- HWANG, CHER WON (Department of Environmental Microbiology, Handong University) ;
- DONG KYU LEE (Department of Biotechnology, Taegu University) ;
- SUN CHEOL KANG (Department of Biotechnology, Taegu University)
- Published : 1998.06.01
Abstract
A ura5 gene encoding Orotate Phosphoribosyl Transferase (OPRTase) of Metarhizium anisopliae was cloned by PCR methods and sequenced. The sequenced ura 5 gene encodes a polypeptide of 234 amino acid residues. This deduced amino acid sequence showed high similarity to other fungi OPRTase and there was no intron sequence between ATG starting codon and TGA ending codon.
Keywords
- Polymerase chain reaction;
- entomopathogenic fungus;
- Metharizium anisopliae;
- orotate phosphoribosyl transferase