• Title/Summary/Keyword: orotate phosphoribosyl transferase

Search Result 2, Processing Time 0.015 seconds

Molecular Cloning and Sequence of URA5 Gene Encoding Orotate Phosphoribosyl Transferase (OPRTase) from Entomopathogenic Fungus, Metarhizium anisopliae

  • HWANG, CHER WON;DONG KYU LEE;SUN CHEOL KANG
    • Journal of Microbiology and Biotechnology
    • /
    • v.8 no.3
    • /
    • pp.284-286
    • /
    • 1998

  • A ura5 gene encoding Orotate Phosphoribosyl Transferase (OPRTase) of Metarhizium anisopliae was cloned by PCR methods and sequenced. The sequenced ura 5 gene encodes a polypeptide of 234 amino acid residues. This deduced amino acid sequence showed high similarity to other fungi OPRTase and there was no intron sequence between ATG starting codon and TGA ending codon.

  • PDF

Isolation and Identification of ura5 Gene in Entomopathogenic Fungus, Metarhizium anisopoliae (살충성곰팡이 Metarhizium anisopliae의 ura5 유전자의 분리동정)

  • Park, In-Cheol;Lee, Dong-Kyu;Kang, Sun-Cheol;Hwang, Cher-Won
    • Applied Biological Chemistry
    • /
    • v.40 no.1
    • /
    • pp.30-33
    • /
    • 1997

  • About 250 bp ura5 gene (Orotate phosphoribosyl transferase) fragment was cloned from genomic DNA of entomopathogenic fungus Metarhizium anisopliae by using PCR method. Entire nucleotide sequences of cloned DNA fragment were determined and analysed as compared with other fungus ura5 genes. The amino acid sequence deduced from the nucleotide sequence showed 85.5% homology to ura5 protein of Trichoderma reesei. Using this 250 bp PCR fragment we have isolated full ura5 gene of M. anisopliae by genomic Southern hybridization and the isolated 4.4 kb DNA fragments were mapped by restrictional enzyme.

  • PDF