Journal of Microbiology and Biotechnology
- 제8권1호
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- Pages.96-100
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- 1998
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- 1017-7825(pISSN)
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- 1738-8872(eISSN)
A Pathway for 4-Chlorobenzoate Degradation by Pseudomonas sp. S-47
- Seo, Dong-In (Department of Microbiology, Chungbuk National University) ;
- Chae, Jong-Chan (Department of Microbiology, Chungbuk National University) ;
- Kim, Ki-Pil (Department of Microbiology, Chungbuk National University) ;
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Kim, Young-Soo
(Department of Pharmacy, Chungbuk National University) ;
- Lee, Ki-Sung (Department of Biology, Pai-Chai University) ;
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Kim, Chi-Kyung
(Department of Microbiology, Chungbuk National University)
- 발행 : 1998.02.01
초록
Pseudomonas sp. S-47 degraded 4-chlorobenzoate (4CBA) to 4-chlorocatechol (4CC) that was subsequently ring-cleaved to form 5-chloro-2-hydroxymuconic semialdehyde. These intermediate compounds were identified by GC-mass spectrometry and UV-visible spectrophotometry. 5-chloro-2-hydroxymuconic acid converted from 5-chloro-2- hydroxymuconic semialdehyde (5C-2HMS) was dechlorinated to produce 2-hydroxypenta-2,4-dienoic acid (2HP-2,4DA) by the strain. These results indicate that Pseudomonas sp. S-47 degrades 4CBA to 2HP-2,4DA via a novel pathway including the meta-cleavage of 4CC and dechlorination of 5C-2HMS.