Journal of Microbiology and Biotechnology
- 제8권1호
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- Pages.92-95
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- 1998
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- 1017-7825(pISSN)
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- 1738-8872(eISSN)
Overexpression of Escherichia coli Thiol Peroxidase in the Periplasmic Space
- Kim, Sung-Jin (Departments of Genetic Engineering, Pai Chai University) ;
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Cha, Mee-Kyung
(Departments of Biochemistry, Pai Chai University) ;
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Kim, Il-Han
(Departments of Biochemistry, Pai Chai University) ;
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Kim, Ha-Kun
(Departments of Genetic Engineering, Pai Chai University)
- 발행 : 1998.02.01
초록
Overproduction of Escherichia coli thiol peroxidase in the periplasmic space was achieved by locating the appropriate gene on a downstream region of the strong T7 promoter. E. coli strain BL21 carrying the recombinant plasmid pSK-TPX was induced by IPTG, lysed, and analyzed by SDS-polyacrylamide gel electrophoresis. A large amount of the overexpressed thiol peroxidase was located in the periplasmic space. A homogeneous thiol peroxidase was obtained from E. coli osmotic shock fluid by simple one-step gel permeation chromatography.
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