Overexpression of Escherichia coli Thiol Peroxidase in the Periplasmic Space

  • Kim, Sung-Jin (Departments of Genetic Engineering, Pai Chai University) ;
  • Cha, Mee-Kyung (Departments of Biochemistry, Pai Chai University) ;
  • Kim, Il-Han (Departments of Biochemistry, Pai Chai University) ;
  • Kim, Ha-Kun (Departments of Genetic Engineering, Pai Chai University)
  • 발행 : 1998.02.01

초록

Overproduction of Escherichia coli thiol peroxidase in the periplasmic space was achieved by locating the appropriate gene on a downstream region of the strong T7 promoter. E. coli strain BL21 carrying the recombinant plasmid pSK-TPX was induced by IPTG, lysed, and analyzed by SDS-polyacrylamide gel electrophoresis. A large amount of the overexpressed thiol peroxidase was located in the periplasmic space. A homogeneous thiol peroxidase was obtained from E. coli osmotic shock fluid by simple one-step gel permeation chromatography.

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