Korean Journal of Plant Tissue Culture (식물조직배양학회지)

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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High Frequency Embryogenesis and Plant Regeneration in Anther Cultures of Pepper

고추 약배양을 통한 고빈도 배발생 및 식물체 재분화

  • 안민영 (생명공학연구소 식물세포 및 분자생물학 Research Unit) ;
  • 김용권 (농협종묘개발센터) ;
  • 민성란 (생명공학연구소 식물세포 및 분자생물학 Research Unit) ;
  • 정원중 (생명공학연구소 식물세포 및 분자생물학 Research Unit) ;
  • 인동수 (생명공학연구소 식물세포 및 분자생물학 Research Unit) ;
  • 유장렬 (생명공학연구소 식물세포 및 분자생물학 Research Unit)
  • Published : 1998.09.01
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Abstract

Culture conditions for high frequency embryogenesis and plant regeneration in anther cultures of various F$_1$ hybrid and homozygous lines of pepper (Capsicum annuum L.) are described. Anthers pigmented less than halfway from the distal end were dissected from the flower bud in which petals elongated 2 mm higher than the receptacle. They were placed on Dumas medium supplemented with 0.1 mg/L 2,4-D and 0.1 mg/L kinetin. After four weeks of culture, embryos began to appear on anthers. After eight weeks of culture, frequencies of embryo formation reached up to 58.3%. Upon transfer to MS basal medium, greater than 95% of embryos developed into plantlets.

여러 계통의 고추(Capsicm annuum L.)의 약을 이용하여 식물체 재분화 조건을 확립하였다. 꽃받침 위로 꽃잎이 2mm 올라온 미성숙 화뢰에서 상부로부터 절반 이하로 착색된 약을 채취하여 0.1mg/L 2,4-D와 0.1mg/L kinetin이 첨가된 Dumas 고체배지에 치상하였다. 배양 4주후부터 배가 발생하였으며, 8주후의 배발생 빈도는 최고 58.3%였다. 배는 MS 기본배지로 옮겨주었을 때 95이상이 발아하여 식물체로 재분화되었다.

Keywords

References

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