Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
권호 표지

Growth Characteristics of Transgenic Potato Using Wild-type Agrobacterium spp.

Agrobacterium spp. 에 의하여 형질전환된 감자조직의 생장특성

  • 양덕춘 (한국인삼연초연구원 유전생리부)
  • Published : 1998.06.01
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Abstract

This study was carried out to obtain the information for growth characteristics of crown gall tumor and hairy root transformed by Agrobacterium spp,. on the media with phytohormones, casein hydrolysate and activated charoal. Crown gall tumors and hairly roots were formed respectively on potato tuber discs infected by tumerfaciens A ch 5 and A.rhizogenes ATCC15834. These tumors and roots could be grown on the phytohormone free media. PCR analysis of Rol C and Vir C gene fragments confirmed that crown gall root was prompted on the medium containing 2,4-D 2mg/l with casein hydrolysate lg/l. The survival ration of crown gall tumor callus derived from potato increased on medium containing the activated charcoal 0.5∼0.2mg/l because of the prevention, on the other hand, hairly roots were necrosis on the same medium. Callus derived from hairly root were excellently grown for a short time by suspension culture on liquid medium containing 2.4-d 2mg/L and casein hydrolysate lg/l.

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References

  1. Plant Physiol v.79 High efficiency transformation of cultured tobacco cells An G.
  2. Amer. J. Bot. v.64 no.2 Crown gall tumorigenesis in potato tuber tissue Anad, V.;Heberlein, K.
  3. Handbook of plant cell culture v.1 vitro production of haploids Bajaj, Y. P. S.;Evans D.A.W.;Sharp, R.;Ammirato, P.V.;Yamada, Y. (eds)
  4. Nature(Lond) v.295 Agrobacterium rhizogenes inserts T-DNA into the genome of the host plant root cells Chilton, M. D.;Tepfer, D. A.;Petit, A.;Tempe, J.
  5. Cell culture and somatic cell genetic of plant v.1 Culture of embryos Collins, G.;Grosser, J.W.
  6. Plant Cell Reports v.7 Genetic transformation of cauliflower(Brassica oleracea L. var Botrytis) by Agrobacterium rhizogenes David, C.;Tempe, J.
  7. Plasmid v.6 Restriction endonuclease mapping of the octopine tumour-inducing plasmid pTiAch5 of Agrobacterium tumefactens De Vos, G.;Beuckeleer, D.;Montagu, M. V.;Schell, J.
  8. Nucleic Acid Res v.19 A simple and rapid method for the preparation of plant genomie DNA for PCR analysis Edwards, K. C.;Johnson, C.;Thompson, C.
  9. Mol Gen Genet v.181 Four new opines from crown gall tumors their detection and properties Ellis, J. G.;Murphy, P. J.
  10. Physiol plant v.34 Effects of activated charcoal on trwoth and morphogenesis in cell cultures q Fridborg, G.;Eriksson, T.
  11. Plant tissue culture methods Callus and cell culture Gamborg, O. L.;Wetter, L. R.;Constable, F.(eds)
  12. Scientia Horticulture v.40 Transformation and regeneration of ornamental kale(Brassica oleracea var. Acephala DC)mediated by Agrobacterium rhizogenes Hosoki, T.;Shiraishi, K.;Kigo. T.;Ando, M.
  13. Mol Gen Genet v.194 Genetic analysis of the individual T-DNA genes of Agrobacterium tumefaciens:further evidence that two genes are involved in indole-3-acetic acid synthesis Inze, D.;Lijsebettens, M. V.;Schell, J.
  14. Korean J. Tissue culture v.15 no.3 Transformation of several plant species with Ri plasmid Lee, J. Y.;Davey, M. R.
  15. Plant Cell Reports v.7 establishment of new axenic hairy root lines by inoculation with Agrobacterium rhizogenes Mugnier, J.
  16. Plant Cell Physiol. v.34 no.5 Effects of the over-expression of the rolC gene on leaf development in transgenic periclinal chimeric plants Oono, Y.;Suzuki, T.;Toki, S.;Ulchimiya, H.
  17. Mol Gen Genet v.190 Petit, A.;David, C.;Dahl, G. A.;Ellis, G. J.;Guyon, P.;Temple, J.
  18. Biotechnology in agriculture and forestry v.3 A gene transfer system for potato(Solamum tuberosum L.) Shahin, E. A.;Simpson, R. B.;Y.P.S. Bajaj(ed)
  19. Appl Environ Microbiol v.61 no.2 PCR detection of Ti and Ri plasmids from phytopathogenic Agrobacteriunm strains Sawada, H.;Ieki, H.;matsuda, I.
  20. Cell culture and somatic cell genetics of plant v.1 Induction and maintenance of embryogenic callus cultures of gramineae Vasil, V.;Vasil, I. K.;Vasil, I. K. (eds)