Korean Journal of Veterinary Pathology (한국수의병리학회지)
- Volume 2 Issue 1
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- Pages.17-24
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- 1998
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- 1226-766X(pISSN)
Expression of c-Jun in pancreatic islet $\alpha$ -cells of nonobese diabetic(NOD) mice
- Park, Sang-Joon (Korea Research Institute of Bioscience & Biotechnology, KIST) ;
- Lee, Sae-Bom (Korea Research Institute of Bioscience & Biotechnology, KIST) ;
- Choi, Yang-Kyu (Korea Research Institute of Bioscience & Biotechnology, KIST) ;
- Lee, Chul-Ho (Korea Research Institute of Bioscience & Biotechnology, KIST) ;
- Hyun, Byung-Hwa (Korea Research Institute of Bioscience & Biotechnology, KIST) ;
- Lee, Keun-Joa (College of Veterinary Medicine, Chungnam National university) ;
- Ryu, Si-Yun (College of Veterinary Medicine, Chungnam National university) ;
- Cho, Sung-Whan (College of Veterinary Medicine, Chungnam National university) ;
- Song, Jae-Chan (College of Veterinary Medicine, Kyungpook National univerity) ;
- Lee, Cha-Soo (College of Veterinary Medicine, Kyungpook National university) ;
- Jeong, Kyu-Shik (Korea Research Institute of Bioscience & Biotechnology, KIST)
- Published : 1998.06.01
Abstract
This is the first report of c-Jun protein expression and mRNA in a pancreatic islet in a nonobese diabetic(NOD) state mice. In this experiment NOD mice with insulin-dependent diabetes mellitus type I at age 16 weeks(n=7) just before death(n=4) were used. The control group consist of prediabetic NOD(8 weeks n=7) and ICR(8 weeks n=7 and 16 weeks n=7) mice. c-Jun positive cells in the pancreatic islet of NOD mice were localized in the same positions as a-glucagon producing cells. immunoreactivity was negative in the prediabetic NOD(8 weeks) and ICR(8 weeks and 16 weeks) mice. The number of c-Jun positive cells in mice with severe diabetic state just before death were significantly decreased when compared to NOD(16 weeks) mice. Expression of c-Jun in mRNA level was assessed by RT-PCR method. The levels of mRNA in NOD(16 weeks) mice group were elevated in total pancreatic tissues. The present results suggest that the induction of proto-oncogene protein may be of significance in assessing cell specific injury and may play a functional role between pancretic islet