KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Characterization and Purification of Agarase from Cytophaga sp. ACLJ-18

한천 분해균(Cytohaga sp. ACLJ-18)이 생산하는 agarase의 정제 및 특성

  • Published : 1998.06.01
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Abstract

Agar degrading enzyme-agarase-was purified from the culture fluid of Cytophaga so/ ACLJ-18, by acetone precipitation, DEAE-Cellulose, Sephadex G-100 and CM-Sephadex C25 column chromatographies. The molecular weight of purified agarase was estimated to be 24,700 dalton by SDS-polyacrylamide gel electrophoresis. The optimum pH and temperature for agarase activity were 7.0 and 40$^{\circ}C$, respectively. this agarase was stable in the pH range of 6.5 - 8.0 and 40$^{\circ}C$, and required 0.35M NaCl for optimum activity. And this agarase was inhibited by metal ions such as Ba2+, Cu2+, Co2+, Mn2+, Hg2+, Zn2+, and showed specificity on agar.

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