• KSBB Journal
• /
• v.13 no.3
• /
• pp.320-324
• /
• 1998

Agar degrading enzyme-agarase-was purified from the culture fluid of Cytophaga so/ ACLJ-18, by acetone precipitation, DEAE-Cellulose, Sephadex G-100 and CM-Sephadex C25 column chromatographies. The molecular weight of purified agarase was estimated to be 24,700 dalton by SDS-polyacrylamide gel electrophoresis. The optimum pH and temperature for agarase activity were 7.0 and 40$^{\circ}C$, respectively. this agarase was stable in the pH range of 6.5 - 8.0 and 40$^{\circ}C$, and required 0.35M NaCl for optimum activity. And this agarase was inhibited by metal ions such as Ba2+, Cu2+, Co2+, Mn2+, Hg2+, Zn2+, and showed specificity on agar.

• KSBB Journal
• /
• v.11 no.5
• /
• pp.593-599
• /
• 1996

The strain which produces agar degrading enzyme was isolated from chiton(Liolophura japonica). The strain was identified as Cytophaga sp. through its morphological, physiological, and biological characteristics. For the production of agar degrading enzyme, 0.3% nutrient broth, 0.2% yeast extract and 0.5% agar was used as nitrogen and carbon source, respectively. The optimal initial pH, NaCl and temperature for the agar degrading activity of Cytophaga sp. were 7.0, 2.0% and $30{\pm}2^{\circ}C$, respectively. Agar degrading activity of enzyme obtained from Cytophaga sp. was increased until the incubation of 96hrs, but after 96hrs, the activity was decreased.