Simultaneous Saccharification and Pervaporative Fermentation of Cellulosic Biomass

투고증발을 이용한 섬유성바이오매스의 동시당화 및 추출발효

  • 공창범 (경원대학교 화학공학과) ;
  • 윤현희 (경원대학교 화학공학과)
  • Published : 1998.02.01

Abstract

Application of pervaporative extraction of ethanol to simultaneous saccharification and fermentation(SSF) of cellulose was investigated. From batch experiments, optimum cellulose substrate and enzyme loadings were found to be 10% and 15 IFPU/g cellulose, respectively. The cellulose conversion was lowered in fed-batch system due to the ethanol accumulation. The activity of the yeast Saccharomyces uvarum used in this study was significantly reduced at ethanol concentrations above around 40 g/L. From pervaporation experiments using PDMS membrane, ethanol was efficiently separated at 38$^\circ C$ and 10 mmHg of a down stream pressure. The pervaporation unit with 240 cm$^2$ of surface area was combined into the SSF reactor. The continuous removal of ethanol by pervaporation during SSF resulted in an improved cellulose conversion. Within the scope of this experiment, ethanol yields in the pervaporative SSF and simple SSF were 68.3% and 56.6%, respectively. The permeate flux for SSF broth pervaporation was about one-half that for the pervaporation of aqueous ethanol solution. Accordingly, the development of a membrane with higher ethanol selectivity and flux will increase the feasibility of this technology.

Keywords

References

  1. Proc. Bioconversion Symp. A Method for Production of Alcohol Directly from Cellulose Using Celluase and Yeast Takaki,M.;S.Abe;S.Suzuki;G.H.Emert;N.Yata;T.K.Ghose(ed.)
  2. Appl. Biochem. Biotech. v.28/29 Simultaneous Saccharification and Fermentation of Pretreated Wheat Straw to Ethanol with Selected Yeast Strains and β-Glucosidase Supplementation Spindler,D.D.;C.E.Wyman;K.Grohmann
  3. J. Ferment. Technol. v.66 Ethanol Production in Simultaneous Saccharification and Fermentation of Cellulose wifh Temperature Profiling Huang,S.Y.;J.C.Chen
  4. AIChE Symp. Ser. v.74 Enzymatic Hydrolysis of Cellulose and Simultaneous Fermentation to Alchol Blotkamp,P.J.;M.Takaki;M.S.Pemberton;G.H.Emert
  5. Biotechnol. Lett. v.3 The Effect of Temperature on the Kinetics of Ethanol Production by Strains of Zymomonas Mobilis Lee,K.J.;M.L.Skotnicki;D.E.Tribe;P.L.Rogers
  6. Biotech. Bioeng. v.26 Simultaneous Saccharitication and Fermentation of Lignocellulosics to Ethanol Under Vacuum Cycling and Step Feeding Ghose,T.K.;P.K.Roychoudhury;P.Ghosh
  7. Biotech. Bioeng. v.49 Simultaneous Saccharification and Extractive Fermentation of Cellulosic Substrates Moritz,J.W.;S.J.B.Duff
  8. Biotechnol. Bioeng. v.36 Ethanol Production from Starch in a Pervaporation Membrane Bioreactor Using Clostrium Themohrosulfuricum Mori,Y.;T.Inaba
  9. Biotechnol. Bioeng. v.38 Continuous Ethanol Production by Immobilized Yeast Reactor Coupled with Membrane Pervaporation Unit Shabtai,Y.;S.Chaimovitz;A.Freeman;E.Katchlski-Katzir
  10. The Selective Recovery of Alcohols from Fermentation Broths by Pervaporation, Separation and Purification Methods v.19 no.1 Hickery,P.J.;C.S.Slater
  11. Appl. Biochem. Biotech. v.51/52 Philippidis,G.P.;T.K.Smith