The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
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Underlying mechanism of antioxidant action of Holotrichia in renal tissues

신장(腎臟) 조직(組織)에서 제조의 항산화(抗酸化) 효과(效果)의 기전(機轉) 연구(硏究)

  • Jeong, Ji-Cheon (Dept. of Internal Medicine, College of Oriental Medicine, Dong-guk University) ;
  • Moon, Sang-Won (Dept. of Internal Medicine, College of Oriental Medicine, Dong-guk University) ;
  • Kim, Kil-Seop (Dept. of Internal Medicine, College of Oriental Medicine, Dong-guk University)
  • 정지천 (동국대학교 한의과대학 내과학교실) ;
  • 문상원 (동국대학교 한의과대학 내과학교실) ;
  • 김길섭 (동국대학교 한의과대학 내과학교실)
  • Published : 1998.08.30
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Abstract

This study was carried out to examine mechanisms by which Holotrichia (HTC) produces protective effect against renal cell injury. HTC extraction (5%) prevented $H_2O_2(50mM)$-induced LDH release and lipid peroxidation in renal cortical slices. When slices were treated with 5% HTC extraction, cellular glutathione content and the superoxide dismutase activity were not altered in control and $H_2O_2$-treated tissues. When slices were treated with 50 mM $H_2O_2$, the catalase activity was significantly inhibited, which was completely restored by addition of 5% HTC. Treatment of slices with 5% HTC extraction increased the glutation peroxidase activity in $H_2O_2$-treated tissues. These results suggest that HTC prevents oxidant-induced cell injury and lipid peroxidation by increasing the activities of catalase and glutathione peroxidase in renal cortical slices.

Keywords

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