Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Electrophoretic pattern and classification of chitinolytic enzymes from Rehmannia glutinosa

지황 chitin 분해효소의 전기영동 양상 및 분류

  • Pan, Cheol-Ho (Department of Agricultural Chemistry and Research Center for New Bio-materials in Agriculture, College of Agriculture and Life Science, Seoul National University) ;
  • Lee, Eun-A (Department of Agricultural Chemistry and Research Center for New Bio-materials in Agriculture, College of Agriculture and Life Science, Seoul National University) ;
  • Jo, Do-Hyun (Department of Biotechnology, Ajou University) ;
  • Kim, Su-Il (Department of Agricultural Chemistry and Research Center for New Bio-materials in Agriculture, College of Agriculture and Life Science, Seoul National University)
  • 판철호 (서울대학교 농업생명과학대학 농화학과 및 농업생물 신소재 연구 센터) ;
  • 이은아 (서울대학교 농업생명과학대학 농화학과 및 농업생물 신소재 연구 센터) ;
  • 조도현 (아주대학교 생물공학과) ;
  • 김수일 (서울대학교 농업생명과학대학 농화학과 및 농업생물 신소재 연구 센터)
  • Published : 1998.12.31
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Abstract

Chitinase, which is one of the pathogenesis-related proteins, was examined from Rehmannia glutinosa. Rehmannia chitinases were classified into basic and acidic groups by chitin affinity column chromatography. According to the chitinase pattern by native PAGE, basic chitinases of low Rf values were extracted more under an acidic condition (pH 2.9) than a basic condition (pH 8.8), while acidic chitinases having high Rf values were mainly detected in basic extracts. There were in total seven chitinase isoforms of three basic and four acidic isoforms in Rehmannia. The range of molecular weight of Rehmannia chitinase was from 28 to 32 kD. It showed the possibility of tissue specific expression of acidic chitinases and basic chitinases were mainly detected in root.

Pathogenesis-related proteins중 chitinase를 지황에서 추출하고 분류하였다. 지황 chitinase는 chitin affinity column chromatography로 염기성 및 산성 두개 군으로 분류되었으며 native PAGE gel상 효소활성을 조사한 결과 이동도가 작은 염기성 chitinase는 pH 2.9의 산성추출 조건에서, 이동도가 큰 산성은 pH 8.8의 염기성추출 조건에서 상대적으로 많이 추출되었다. 지황에는 염기성 3개, 산성 4개등 총 7개의 chitinase isoform이 존재하며 이들의 분자량은 $28{\sim}32\;kD$내외이고 지상부와 달리 뿌리에는 주로 염기성 chitinase만 검출되어 일부 산성효소는 조직 특이적으로 발현될 가능성을 보여주고 있다.

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