Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Isolation and Characterization of 4-Hydroxy-3-methoxycinnamic Acid and 3,4-Dihydroxycinnamic Acid with Antimicrobial Activity from Root of Pulsatilla koreana

백두옹에서 항미생물 활성을 갖는 4-Hydroxy-3-methoxycinnamic Acid와 3,4-Dihydroxycinnamic Acid의 분리 및 동정

  • Lee, Hyang-Hee (Department of Food Science and Technology, Chonnam National University) ;
  • Ma, Seung-Jin (Department of Food Science and Technology, Chonnam National University) ;
  • Moon, Jae-Hak (Department of Nutrition, University of Tokushima) ;
  • Park, Keun-Hyung (Department of Food Science and Technology, Chonnam National University)
  • Published : 1998.04.30
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Abstract

The MeOH extract from root of Pulsatilla koreana was showed antimicrobial activities against bacteria and yeast. The antimicrobial active substances of MeOH extract were successfully purified with solvent fractionation, silica gel adsorption column chromatography and Sephadex LH-20 column chromatography. The purified two active substances were isolated by HPLC and identified as 4-hydroxy-3-methoxycinnamic acid and 3,4-dihydroxycinnamic acid by MS, $^{1}H-NMR$ and $^{13}C-NMR$.

할미꽃 $(Pulsatilla\;koreana\;N_{AKAI})$ 뿌리인 백두옹의 MeOH 추출물이 세균과 효모 등의 미생물에 대해 항미생물 활성을 나타내자 백두옹에 함유된 항미생물 활성물질의 탐색을 시도하였다. MeOH 추출물을 생물검정법을 지표로 solvent fractionation, silica gel adsorption column chromatography, Sephadex LH-20 column chromatography 등으로 정제하고, HPLC에 의해 활성물질을 분리하였다. 분리된 두 활성물질은 MS, $^{1}H-NMR$, $^{13}C-NMR$에 의해 4-hydroxy-3-methoxycinnamic acid와 3,4-dihydroxycinnamic acid로 동정되었다.

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