Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Development of Anticancer Agents from Korean Medicinal Plants. Part 6. -Cytotoxic Activity of the Ethyl Acetate Soluble Fraction of Lonicerae flos against Human Oral Epitheloid Carcinoma Cells-

한국산 생약으로부터 항암물질의 개발(제6보). -금은화 Ethyl Acetate 가용성 분획의 인체 구강유상피암종세포에 미치는 세포독성작용-

  • Han, Du-Seok (Department of Oral Anatomy, School of Dentistry, Wonkwang University) ;
  • Baek, Kyong-Hyun (Department of Oral Anatomy, School of Dentistry, Wonkwang University) ;
  • Kim, Young-Ok (Department of Chemistry, College of Natural Sciences, Wonkwang University) ;
  • Choi, Kyu-Eun (Department of Chemistry, College of Natural Sciences, Wonkwang University) ;
  • Kwag, Jung-Suk (Department of Dental Hygenic, Mokpo College) ;
  • Baek, Seung-Hwa (Department of Chemistry, College of Natural Sciences, Wonkwang University)
  • 한두석 (원광대학교 치과대학 구강해부학교실) ;
  • 백경현 (원광대학교 치과대학 구강해부학교실) ;
  • 김영옥 (원광대학교 자연과학대학 화학과) ;
  • 최규은 (원광대학교 자연과학대학 화학과) ;
  • 곽정숙 (목포전문대학 치위생과) ;
  • 백승화 (원광대학교 자연과학대학 화학과)
  • Published : 1998.03.30
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Abstract

This study was carried out to develop antitumor agents based on effects of the ethyl acetate soluble fraction of the methanolic extract of Lonicerae flos on human oral epitheloid carcinoma cells. Human oral epitheloid carcinoma cells were cultured in RPMI-1640 media containing 10% fetal bovine serum, antibiotic, and fungizone. After incubation for 24 hrs, the cells were treated with A, B, C, D, and E fractions for 48hrs under the same condition. MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazoliumbromide), NR (Neutral red) and SRB (Sulforhodamine B protein) assay were performed. The light microscopic findings were observed by inverted microscope. In MTT assay, fraction B was shown significant antitumor activity (P<0.001), fraction E was shown significant antitumor activities (P<0.05), but the other fractions were not shown. In NR assay, fraction B was shown significant antitumor activity (P<0.001). In SRB assay, fractions B was shown significant antitumor activities (P<0.01). fractions A and D were shown significant antitumor activities (P<0.05). but the other fractions were not shown. In light microscopy. the fraction B of the ethyl acetate soluble fraction of the methanolic extract of Lonicerae flos showed the highest antitumor activity. These finding suggested that fraction B possessed the most antitumorous agent.

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