한국균학회지 (The Korean Journal of Mycology)

한국균학회 (The Korean Society of Mycology)
권호 표지

사과탄저병균(Glomerella cingulata)에 대한 Pseudomonas sp. DGUM 5051의 항진균 활성

Antifungal Activity of Pseudomonas sp. DGUM 5051 Against Apple Bitter-rot Causing Fungus, Glomerella cingulata

  • 김정미 (동국대학교 교육대학원 생물교육) ;
  • 이민웅 (동국대학교 생명자원과학대학 응용생물학과) ;
  • 한영환 (동국대학교 자연과학대학 생물학과)
  • Kim, Jung-Mi (Major of Biology Education, Graduate School of Education, Dongguk Univ.) ;
  • Lee, Min-Woong (Department of Applied Biology, College of Life Resources Science, Dongguk Univ.) ;
  • Han, Yeong-Hwan (Department of Biology, College of Natural Science, Dongguk Univ.)
  • 발행 : 1998.12.30
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초록

사과탄저병균, Glomerella cingulata에 선택적으로 항균활성이 있는 세균을 분리한 후 형태 및 생리화학적인 특성을 기준으로 동정하여 Pseudomonas sp. DGUM 5051로 명명하였다. 분리균의 생장을 위한 최적 pH와 온도는 각각 6.0과 $30^{\circ}C$이었고, 최적 항균활성을 나타내는 pH와 온도는 각각 7.0와 $24^{\circ}C$이었다. 사용한 복합배지 중 생장 및 항균활성에 있어서는 Brucella, Brain heart infusion과 LB배지가 우수하였다. Mineral salts 배지에 유일 탄소원, 질소원 및 인산원으로 각각 Sucrose, $KNO_3$ 및 $K_2HPO_4$를 첨가하였을 때 우수한 항균활성을 나타내었다. Pseudomonas sp. DGUM 5061의 항균활성은 배양 12시간 경과 후에 나타났다.

The strain DGUM 5051, an antagonistic bacterium against apple-bitter rot causing Glomerella cingulata, was isolated from soil in Kyongju. Based on the morphological and physiological characteristics, the bacterium was identified as Pseudomonas sp. and named as Pseudomonas sp. DGUM 5051. The optimal pH and temperature for cell growth were pH 6.0 and $30^{\circ}C$, whereas those for antifungal activity were pH 7.0 and $24^{\circ}C$, respectively. Among the complex media tested, brucella medium, brain heart infusion medium and Luria-Bertani medium were good for both cell growth and antifungal activity. The high antifungal activity was found in the mineral salts medium, in which sucrose, $KNO_3$ and $K_2HPO_4$ were used as sources of carbon, nitrogen and phosphorus, respectively.

키워드

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