• Korean Journal Plant Pathology
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• v.14 no.6
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• pp.654-660
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• 1998

Colletotrichum gloeosporioides and Glomerella cingulata are the most important pathogens causing anthracnose which may reduce the stand rate and yield on wide kinds of plants including strawberry. Average occurrence rate of anthracnose is 36.9% on major strawberry cropping areas in Korea. We newly found that C. gloeosporioides which does not forming a sexual stage, infects strawberry and differs in some characteristics concerning virulence, cultural and morphological properties to G. cingulata which has a sexual stage. C. gloeosporioides was mainly isolated from the crown with 35.2% rate, while G. cingulata was largely isolated from petiole, runner with 40.9% rate in infected strawberry plants. These two pathogens showed significant differences in cultural characteristics such as perfect stage formation, temperature response as well as benomyl resistance. It was demonstrated that C. gloeosporioides has significantly stronger pathogenicity than G. cingulata in pathogenicity test carried on strawberry plants to various strawberry cultivars. Akihime, Akaneko and Nyoho forcing cultured strawberry cultivars, considered to be susceptible, while semiforcing cultured cultivars, such as Suhong and Holowase, were shown resistant to both pathogens. In non-wound inoculation, C. gloeosporioides was shown pathogenicity on the apple fruit, but G. cingulata could not infect it.

• The Plant Pathology Journal
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• v.18 no.4
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• pp.228-230
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• 2002

Anthracnose symptoms caused by Glomerella cingulata were observed on leaves and stems of gardenia in Sunchon, Jeonnam in Korea in 2000. Symptoms on infected plants typically appeared as irregularly circular, dark-brown ring spots and water-soaked brown lesions. Based on cultural and morphological characteristics, the fungus (G-00-03 isolate) from the diseased plants was identified as Colletotrichum gloeosporioides, and its teleomorph stage was Glomerella cingulata. Healthy gardenia artificially inoculated with fungal spores showed anthracnose symptom 7 days after inoculation. This is the first report of gardenia anthracnose caused by Glomerella cingulata in Korea.

• Korean Journal Plant Pathology
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• v.10 no.2
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• pp.119-122
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• 1994

Effects of organophosphorus fungicides on cutinase activity from Glomerella cingulata causing apple anthracnose and infection of apple were investigated. Diisoprophylfluorophosphate (DFP) inhibited the enzyme activity indicating that catalysis involves an active serine. In inhibition of the enzyme activity by organophosphorus fungicides, I50 (molar concentration of fungicide at which the enzyme activity is inhibited 50%) of Hinosan and Kitazin P were 26.3 $\mu$M and 427.7 $\mu$M, respectively. At concentration of 10-3 M DFP and organophosphorus fungicides, the infection of G. cingulata was inhibited to 5% in comparison with 15% infection at the unwounded healthy control, but increased to 30% when added with 1 mg/ml of cutinase. Mycelial growth was 36 mm in colony diameter on the medium added with 10-4M of hinosan in comparison with 90 mm of the untreated control, but was 90 mm on the medium added with 10-4M of kitazin P showing lower inhibition than hinosan. The spore germination was more than 60% at all the concentrations of both fungicides.

• Korean Journal Plant Pathology
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• v.10 no.2
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• pp.148-150
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• 1994

Anthracnose of statice (Limonium sp. var. Misty Blue and L. sinuatum Mill. cv. Early Blue) occurred up to 20% in vinyl-houses at Buan, Imsil and Namwon from July to November, 1993. All isolates from the anthracnose symptoms were identified as Glomerella cingulata (Stonem.) Spauld. & Schrenk and its anamorph was Colletotrichum gloeosporioides (Pens.) Sacc. Pathogenicity of the isolates was ascertained on the statice host by artificial inoculation. The symptoms on leaves appeared as circular brown spots in early stage and developed to zonate spots. The spot aggregated and diseased leaves were blighted. The symptom also developed on the stems of severely infected plants and the infected branches were blighted. A lots of acervuli developed on the lesions of stems. Acervuli on necrotic lesions were setose, rounded and elongated. Setae variable in length, 1~4 septate, brown, slightly swollen at the base and tapered to the apex on which conidia were occasionally borned. Statice was revealed as the new host of G. cingulata.

• Korean Journal Plant Pathology
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• v.12 no.4
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• pp.395-401
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• 1996

국내의 고추, 사과, 포도, 스타티스(statice)에서 분리한 Glomerella cingulata 균주들의 병원성과 체세포화 합성군(vegetative compatibility group : VCG)과의 관계를 조사하였다. 사과, 포도, 스타티스에서 분리된 균주들은 전기주에 모드 병원성을 나타내었으나 고추에는 병원성이 없었다. 한편, 고추에서 분리된 균주들은 무상처접종시 병원성을 나타내지 못하였다. Nitrate nonutilizing(nit) mutant는 1.5%의 KClO\ulcorner을 첨가한 최소배지에서 선발하였다. nit mutnat들은 nit1, nit2, nit3, nitM의 4가지 표현형 중 하나에 속하였다. VCG는 표현형이 다른 nit mutan간의 대치배양을 통해 결정하였는데 사과, 포도, 스타티스에서 분리한 균주들간에는 같은 VCG를 나타낸 반면 고추에서 분리된 균주들은 다른 VCG로 나타났다. 따라서 G. cingulata 균주들의 체세포화합성은 병원성과 밀접하게 관련되어 있음을 알수 있었다. 또한 동일한 VCG에 속하며 기주가 다른 균주들의 대치배양시 heterokaryon을 형성했으나 자낭을 형성하지는 않았다.

• Research in Plant Disease
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• v.10 no.3
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• pp.198-202
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• 2004

The overwinter and survival of Colletotrichum gloeosporioides and Glomerella cingulata in strawberry tissues under field conditions was investigated in 2001 and 2002. The rates of overwinter survival in plant petiole, runner and crown were 68.7,14.3, and 26.7％, respectively. But, under field condition, viable conidia of overwinter were not detected at the surface, 3, 5, and 10 cm depths of soil. To investigate the survival ability in soil and plant debris, C. gloeosporioides and G. cingulata isolates from strawberry were examined in laboratory and field. The viability of conidia was rapidly declined in all the treatments, with a 40％ reduction in population within 10 days. In soil, the survival ability of G. cingulata conidia was similiar to that of C. gloeosporioides. The survival rate of conidia was highest under cool and dry soil conditions, and was decreased by increasing both soil temperature and moisture content. Similar results were also obtained under the condition that infected petioles were buried in soil. Results suggested that conidia as well as plant debris might be a main primary inoculum source of strawberry anthracnose.

• Korean Journal Plant Pathology
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• v.10 no.2
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• pp.105-111
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• 1994

Pathogenic fungal species associated with apple anthracnose were studied with diseased fruits collected over two major apple growing areas from 1986 to 1988. Colletotrichum acutatum Simmonds, c. gloeosporioides(Penz.) Sacc. and Glomerella cingulata(Stonem.) Spauld. & Schrenk were identified based on their mycological characteristics. C. acutatum could be easily differentiated from C. gloeosporioides by producing their fusiform conidia and developing apricot∼salmon colony colors, non-forming an ascigerous stages. Growth rate of C. acutatum were slow in plate culture. The three species of anthracnose fungi tested caused apple rot. Symptoms produced by C. gloeosporioides were larger than those of the others. C. gloeosporioides and G. cingulata produced rot lesions on apples that appeared became brown and later black, but C. acutatum produced rot lesions appeared yellowish brown and later reddish brown. G. cingulata has the major species responsible for apple anthracnose, epidemics in Korea.

• The Plant Pathology Journal
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• v.17 no.4
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• pp.236-241
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• 2001

Serve outbreaks of anthracnose were observed on perilla plants grown in greenhouses and open fields in several locations in Korea during the disease survey from 1997 to 2000. A total of 53 isolates of Colletotrichum spp. and Glomerella sp. was obtained from diseased perilla plants and identified based on their morphological and cultural characteristics. Forty isolates were identified as Colletotrichum gloeosporioides, three isolates as C. coccodes, five isolates as C. dematium, and the other five isolates as Glomerella cingulata, the teleomorph of C. gloeosporioides. All isolates of C. gloeosporioides tested by artificial inoculation were strongly virulent on perilla plants, but isolates of the other species were weakly or not virulent. Anthracnose symptoms induced on the perilla plants by artificial inoculation with the isolates of C. gloeosporioides were similar to those observed in the fields. This study revealed that C. gloeosporioides is the main causal fungus of perilla anthracnose.

• The Plant Pathology Journal
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• v.16 no.5
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• pp.294-296
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• 2000

Anthracnose symptoms were frequently found on stems of Indian fig cactus in Cheju island of Korea in 1998 and 1999. Typical symptoms were gray to black dry rot of stems with concentric arrays of dot-like spots. A Glomerella sp. or a Colletotrichum sp. was frequently isolated from the symptoms, both of which were identified as Glomerella cingulata and its anamorph, Colletotrichum gloeosporioides based on their morphological and cultural characteristics. Out of 31 isolates obtained from the symptoms, 12 isolates were the anamorph producing only conidia, four isolates the telemorph producing only ascospores, and 15 isolates the holomorph producing both spores on potato dextrose agar. Stem rots similar to the original anthracnose symptoms were induced by wound inoculation of conidia and ascospores but not by non-wound inoculation. The anamorphic isolates caused more extensive stem rot than the telemorphic and holomorphic ones.

• The Korean Journal of Mycology
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• v.23 no.2 s.73
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• pp.114-120
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• 1995

Colletotrichum dematium, C. gloeosporioides and Glomerella cingulata were detected in seed samples collected from diseased red pepper (Capsicum annuum) using blotter method. C. gloeosporioides was the predominant species in seed samples tested and followed by C. dematium and G. cingulata. When the seed components were plated C. dematium, C. gloeosporioides and G. cingulata were detected from seed coat, endosperm and cotyledon. The three anthracnose fungi were recorded more frequently from seed coat than that of observed in the endosperm and cotyledon. Seed infection with C. dematium, C. gloeosporioides and G. cingulata caused seed rotting, damping off and seedling blight of red pepper plants. According to the inoculation experiments, it was shown that C. gloeosporioides was the most virulent among three species. C. dematium showed weak virulence when the plants were wounded, and G. cingulata was wound parasite or weakly virulent on red fruits. Benlate T (benomyl+thiram) and Homai (thiophnate-methyl+thiram) were effective to anthracnose fungi when treated to infected seeds.