초록
영지의 육종에 필요한 단핵균주를 얻기 위하여 시험한 결과 포자채취는 자실체를 수확하지 않은 상태에서 채취하는 것이 양호하였다. 영지의 포자 발아는 G. lucidum ASI 7004균주는 PDA배지를 이용하여 $25{\sim}30^{\circ}C$에서 발아시켰을 때 0.02%였으며 ASI 7091 균주는 $30^{\circ}C$에서 0.05%였다. G. oregonense ASI 7067은 PDA 배지에서 $35^{\circ}C$로 배양하였을 때 0.67%로 가장 높았다. G. lucidum의 경우는 단핵균주의 교배형을 결정하지 못하였으나, G. oregonense은 $A_1B_1,\;A_1B_2,\;A_2B_1,\;A_2B_2$의 뚜렷한 4극성으로 나타났다.
A study was carried out to explore the method for the germination of the spore of Ganoderma. Three strains of Ganoderma were examined using 2 different media such as PDA and LBA at three different temperatures. As it has been known the germination rate was very low under all the conditions examined. G. lucidum ASI 7004 showed 0.02% germination on both media at $25^{\circ}C$ and $30^{\circ}C$ respectively. The germination rate of ASI 7091 was 0.05% on PDA medium at $30^{\circ}C$. The germination rate of G. oregonense ASI 7067 was 0.67% on PDA at $35^{\circ}C$. LBA medium was found to be inadequate for the germination of Ganderma species in this study. In a consequent study, four mating types of G. oregonense such as $A_1B_1,\;A_1B_2,\;A_2B_1\;and\;A_2B_2$ were identified.