The Journal of Korean Society of Virology (대한바이러스학회지)
- Volume 27 Issue 1
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- Pages.87-95
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- 1997
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- 1225-2344(pISSN)
Screening of Anti-HIV-1 Activity of Natural Products by MTT Assay
MTT Assay에 의한 천연물질의 항 HIV-1 활성 검색
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Lee, Joo-Shil
(Center for AIDS Research, Department of Viral Diseases, National Institute of Health) ;
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Nam, Jeong-Gu
(Center for AIDS Research, Department of Viral Diseases, National Institute of Health) ;
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Kang, Chon
(Center for AIDS Research, Department of Viral Diseases, National Institute of Health) ;
- Lee, Hong-Rae (Center for AIDS Research, Department of Viral Diseases, National Institute of Health) ;
- Lee, Young-Jong (Center for AIDS Research, Department of Viral Diseases, National Institute of Health) ;
- Shin, Yung-Oh (Center for AIDS Research, Department of Viral Diseases, National Institute of Health)
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이주실
(국립보건원 바이러스질환부 면역결핍연구실) ;
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남정구
(국립보건원 바이러스질환부 면역결핍연구실) ;
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강춘
(국립보건원 바이러스질환부 면역결핍연구실) ;
- 이홍래 (국립보건원 바이러스질환부 면역결핍연구실) ;
- 이영종 (국립보건원 바이러스질환부 면역결핍연구실) ;
- 신영오 (국립보건원 바이러스질환부 면역결핍연구실)
- Published : 1997.06.30
Abstract
Methanol and/or boiling water extraction of 201 natural products and subsequent MTT assay using MT-4 cell line was carried out to screen the anti-HIV-1 activity. Among 97 methanol extracts, 7 extracts from Chrysanthemi Indicium Flos, Magnoliae Cortex, Machili Cortex, Reynoutriae Rhizoma, Lithospermi Radix, Agastachis Herba, and Chaenomelis Fructus showed anti-HIV-1 activity and their SI value were 2.25 to 5.77. In addition, among 119 boiling water extracts, 10 extracts from Lonicerae Caulis et Foloium, Elsholtziae Herba, Leonuri Herba, Portulacae Herba, Schizonepetae Herba, Curcumae Rhizoma, Amomi Cardamomi Fructus, Cirsii Radix et Herba, Carpesii Herba, and Siegesbeckiae Herba showed anti-HIV-1 activity and their SI value were 1.30 to 7.64. Methanol extracts of above seven natural products were fractionated and the anti-HIV-1 activity of each fraction was examined. Extraction was carried out with hexane, chloroform, butanol, and water to trace active anti-HIV-1 componets. As a result, the water fraction of Magnoliae Cortex, Machili Cortex, Reynoutriae Rhizoma, Agastachis Herba, Chaenomelis Fructus and the butanol fraction of Chrysanthemi Indicium Flos, Reynoutriae Rhizoma showed anti-HIV-1 activity and their SI value were 1.40 to 8.02. We could reach a conclusion that studies to trace the anti-HIV-1 active component of each natural products in further fractionation and to identify its structure by Infrared spectroscopy, NMR spectroscopy and gel permeation chromatography were needed.