The Journal of Korean Society of Virology (대한바이러스학회지)

The Korean Society of Virology (대한바이러스학회)
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Study on the Zidovudine Resistance of HIV-1 Isolated Strains in Korea

국내 HIV-1 분리주에 대한 Zidovudine의 저항성 연구

  • Nam, Jeong-Gu (Center for AIDS Research, Department of Viral Disease, National Institute of Health) ;
  • Kang, Chon (Center for AIDS Research, Department of Viral Disease, National Institute of Health) ;
  • Lee, Joo-Shil (Center for AIDS Research, Department of Viral Disease, National Institute of Health) ;
  • Lee, Hong-Rae (Center for AIDS Research, Department of Viral Disease, National Institute of Health) ;
  • Shin, Dong-Yun (Center for AIDS Research, Department of Viral Disease, National Institute of Health) ;
  • Park, Yong-Keun (Department of Biology, the Graduated School, Korea University) ;
  • Shin, Yung-Oh (Center for AIDS Research, Department of Viral Disease, National Institute of Health)
  • 남정구 (국립보건원 바이러스질환부 면역결핍연구실) ;
  • 강춘 (국립보건원 바이러스질환부 면역결핍연구실) ;
  • 이주실 (국립보건원 바이러스질환부 면역결핍연구실) ;
  • 이홍래 (국립보건원 바이러스질환부 면역결핍연구실) ;
  • 신동윤 (국립보건원 바이러스질환부 면역결핍연구실) ;
  • 박용근 (고려대학교 생물학과 대학원) ;
  • 신영오 (국립보건원 바이러스질환부 면역결핍연구실)
  • Published : 1997.06.30
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Abstract

To examine AZT resistance of HIV-1 isolates from AZT treated or untreated Korean, several biological characteristics such as syncytium formation, HIV-1 reverse transcriptase activity and the p24 antigen production in MT-2 cells infected with 4 HIV-1 isolates were determined. As controls, we tested HIV-1 HTLV-IIIB and pre-drug isolate as AZT susceptible strains, in addition to HIV-1 RTMC/MT-2 and post-drug isolate as AZT resistant strains. When the inoculum size of HIV-1 was 300 $TCID_{50}$/well and 100 $TCID_{50}$/well, the AZT susceptibility of AZT untreated HIV-1 isolates 8806 and 9571 were similar to that of HIV-1 HTLV-IIIB and AZT-susceptible HIV-1 strains. When we evaluated AZT resistance of isolates HIV-1 8812 and 9113 treated with AZT for 36 months by observation of syncytium formation, HIV-1 8812 showed resistance simillar to that of HIV-1 RTMC/MT-2 strain forming syncytium up to AZT $1{\mu}g/ml$, and HIV-1 9113 showed resistance identical with that of AZT-resistant HIV-1 strain which formed syncytium up to AZT $10\;{\mu}g/ml$. Especially, when we evaluated AZT resistance by HIV-1 reverse transcriptase activity and the p24 antigen production, HIV-1 isolates 8812 and 9113 showed much higher resistance (>10 - 200 fold) compared with HIV-1 RTMC/MT-2 and AZT-resistant HIV-1 strain.

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