식물조직배양학회지 (Korean Journal of Plant Tissue Culture)

한국식물생명공학회 (The Korean Society of Plant Biotechnology)
권호 표지

꿩의비름(Sedum erythrostichum Miq.)의 잎과 줄기 절편으로부터 식물체의 재분화에 미치는 생장조절제의 영향

Effect of Plant Growth Regulators on Plant Regeneration from Leaf and Stem Explant Cultures of Sedum erythrostichum Miq.

  • 윤의수 (공주대학교 자연과학대학 생물학과)
  • 발행 : 1997.09.01
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초록

꿩의비름(Sedum-erythrostichum Miq.)의 잎과 줄기의 절편을 몇 가지의 호르몬이 첨가된 MS 배지에 배양하였다. 배양 2주후 2.0 mg/L. 2,4-D와 1.0 mg/L BA를 혼합한 배지에서 잎의 절편으로부터 100%의 캘러스가 형성되었다. 이 캘러스를 2.0 mg/L 2,4-D와 1.0 mg/L BA를 혼합한 배지로 계대배양하였을때 캘러스의 생장이 왕성하였다. 또한 2.0 mg/L NAA와 1.0 mg/L BA를 혼합한 배지로 계대배양한 캘러스로부터 부정아의 분화가 왕성하였다. 생장호르몬이 없는 배지에서 분화된 신초는 1.0 mg/L IAA를 첨가한 배지에서 발근하여 완전한 식물체로 생장하였다.

Leaf and stem explants of Sedum erythrostichum Miq. were cultured on MS medium supplemented with various combinations of growth regulators. After two weeks of culture, 100% of the leaf explants formed calli on medium containing 2.0 mg/L 2, 4-D and 1.0 mg/L BA. Callus proliferated when subcultured on medium containing 2.0 mg/L 2, 4-D and 1.0 mg/L BA. Numerous adventitious buds were regenerated from callus cultured on the medium containing 2.0 mg/L NAA and 1.0 mg/L BA. Root formation from shoot was occurred on the MS basal medium containing 1.0 mg/L IAA.

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참고문헌

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