식물조직배양학회지 (Korean Journal of Plant Tissue Culture)

한국식물생명공학회 (The Korean Society of Plant Biotechnology)
권호 표지

기내 사과나무 조직의 신초 재분화에 미치는 생장조절물질의 영향

Influence of Growth Regulators on Adventitious Shoot Regeneration from Tissues of Malus domestica cv. 'Gala' in vitro

  • 발행 : 1997.05.01
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초록

'Gala' 잎조직의 신초 재분화에 효과적인 cytokinin 종류와 농도는 BA 4.0 ㎎/L였고, auxin 종류와 농도는 IAA, IBA, 및 NAA 각각 0.1 ㎎/L였다. BA와 NAA를 농도별로 조합 처리하여 'Gala'의 잎, 절간 및 엽병의 신초 재분화 정도를 비교한 결과, 잎조직은 0.5 ㎎/L NAA + 6.0 ㎎/L BA와 0.5㎎/L NAA + 0.8 ㎎/L BA 처리구에서, 절간조직은 0.1 ㎎/L NAA + 0.8 ㎎/L BA와 1.0 ㎎/L NAA + 0.8 ㎎/L BA 처리구에서 신초 재분화율이 가장 높게 나타났으며, 엽병조직은 0.1 ㎎/L NAA + 4.0 ㎎/L BA 처리구에서 가장 높았다.

The most effective cytokinin source for adventitious shoot regeneration of in vitro grown leaves from 'Gala' apple was BA with the concentration of 4.0 mg/L, while auxin source was IAA, IBA and NAA with the concentration of 0.1 mg/L, respectively. As the result of combinational treatment of BA and NAA, 6.0 or 8.0 mg/L BA with 0.5 mg/L NAA was effective for adventitious shoot regeneration from leaf tissues of 'Gala', 0.1 mg/L NAA + 8.0 mg/L BA and 1.0mg/L NAA + 8.0 mg/L BA for internode, and 0.1 mg/L NAA + 4.0 mg/L BA for petiole.

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참고문헌

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  2. Adv Hort Sci v.1 In vitro regeneration from somatic tissues and seed explants of apple Barbieri, C.;S. Morini
  3. Acta Horticulturae v.280 Improving yield of adventitious shoot in apple Dufour, M
  4. Acta Horticulturae v.280 Cultivar dependent responses to regenration from leaves in apple Fasolo, F.;S. Predieri
  5. J Plant Physiol v.132 Factors affecting high frequency plant regeneration from apple leaf tissues cultured in vitro James,D.J.;A.J. Passey;E.Rugini
  6. J Hort Sci v.59 Plant regeneration from callus tissue cultures of the cherry rootstock Colt(Prunus anium x P. pseudocersus)and the apple rootstock M25(Malus pumila) Jones,O.P.;J.A. Gayner;R. Watkins