초록
나팔수선 킹알프레드의 화경배양에서 얻은 대량의 소자구를 비대시키기에 적합한 호르몬 농도, 저온처리기간, 저온 처리 후 구비대에 적합한 호르몬 농도를 구명코자 하였다. 분화된 식물체의 구비대 및 생육은 BA 2.5mg/L에 NAA 2.5mg/L이 첨가된 MS배지에서 높았다. 자구비대를 위한 저온처리 기간은 8주간이 좋았으며 저온처리후 배양은 NAA 1mg/L가 첨가된 배지가 구비대에 가장 효과적이었다.
This experiments were conducted to investigate growth regulator concentrations and a period of chilling treatment for increasing the size of bulblets of Narcissus pseudo-narcissus cv King Alfred. Combination of 2.5 mg/L BA and 2.5 mg/L NAA remarkedly increased the diameter and growth of regenerated bulblets. Chilling period favorable for the growth of regenerated bulblets was found to be 8 weeks, and addition of 1.0 mg/L NAA alone to MS medium was the most effective for increasing the size of bulblet for the culture after the chilling treatment.