한국동물위생학회지 (Korean Journal of Veterinary Service)
- 제20권2호
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- Pages.195-203
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- 1997
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- 3022-7372(pISSN)
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- 2287-7630(eISSN)
소 Coronavirus 분리에 관한 연구
Isolation of bovine Coronavirus from calves with diarrhea
초록
Fecal samples were collected from 47 calves with diarrhea and 12 clinically normal co-h-abitants, and tested for virus using MDBK cell cultures. Three cytopathic viruses were isolated from 8 fecal samples obtained from diarrheic calves. The isolated viruses were neutralized by bovine coronavirus hyperimmune serum In plaque reduction assay and were detected in the cytoplasm of MDBK cell by bovine coronavirus hyperimmune serum using immunofluorescence staining. The viruses agglutinated mouse erythrocytes only among the various animal erythrocytes tested and new isolates were identified as bovine coronavirus.