Dietary Docosahexaenoic Acid Decreases Plasma Triglycerides with Mixed Effects on the Indices of $\beta$-oxidation

  • Cha, Youn-Soo (Department of Food and Nutrition Yosu National Fisheries University)
  • Published : 1997.11.01

Abstract

One known effect of long chain n-3 polyunsaturated fatty acids is their ability to decrease plasma triglycerides. However, identification of the specific n-3 fatty acids and the underlying mechanisms responsible for this change remains uncertain. This present study was designed to evaluate the effects of moderate levels of dietary docosahexaenoic acid (22 :6(n-3)) on modulating plasma triglyderides. Male CD-1 mice were maintained for 15 days on identical diets containing either docosahexahexaenoic acid ethyl ester(1.5%, w/w) or linoleic acid(18 : 2(n-6)) ethyl ester (1.5%, w/w) . Plasma triglycerides were 40% lower in the docosahexaenoic acid group than in the linoleic acid group. Hepatic carnitine palmitoyltransferase activity (a key regulatory enzyme for mitocondria $\beta$-oxidation) was not significantly different between the dietary groups. However, plasma acid soluble acylcarnitine levels (which increase with increasing $\beta$-oxidation )were significantly higher in the decosahexaenoic acid group. This data suggests that plasma triglyceride levels are lower in mice fed diets containing moderate levels of docosahexaenoic acid compared to linoleic acid, but this effect on plasma triglycerides is not modulated through an augmentation of mitochondrial $\beta$-oxidation.

Keywords

References

  1. In Atherosclerosis Review v.22 Austin,M.A.;A.M.Gotto(ed.);R.Paoletti(ed.)
  2. J Lipid Res v.30 Fish oils and plasma lipids and lipoprotein metabolism in humans : A critical review Harris,W.S.
  3. Annu Rev Nutr v.10 Effects of n-3 fatty acids on lipid metabolism Nestel,P.J.
  4. Nutr Rev v.50 How does fish oil lower plasma triglycer-ides? Coniglio,J.G.
  5. Biochim Biophys Acta v.1126 Evidence for mechanisms of the hypotriglyceridemic effect of n-3 polyunsaturated fatty acids Surette,M.E.;Whelan,J.;Broughton,K.S.;Kinsella,J.E.
  6. J Nut. Sci Vitaminol v.30 Differential effects of dietary eicosapentaenoic and docosahexaenoic fatty acids on lowering of triglyceride and cholesterol levels in the serum of rats on hypercholesterolenic diets Kobatake,Y.;Kuroda,K.;Jinnouchi,H.;Nishide,E.;Inname,S.
  7. Lipids v.24 Feeding pure docosahexaenoate or arachidonate decreases plasma tricaylglycerol secretion in rats Williams,M.A.;Tinoco,J.Yang,Y.T.;Bird,M.I.;Hincenbergs,I.
  8. Lipids v.25 On the effect of peroxisomal β-oxidation and carnitine palmitoyltransferase activity by eicosapentaenoic acid in liver and heart from rats Aarsland,A.;Lundquist,M.;Borretsen,B.;Berge,R.K.
  9. Am J Clin Nutr v.55 Habitual fish consumption, plasma phospholipid fatty acids, and serum lipids : The Trφmso Study Bonna,K.H.;Bjerve,K.S.;Nordoy,A.
  10. J Lipid Res v.34 Docosahexaenoic acid shows no triglyceride-lowering effects but increases the peroxisomal fatty acid oxidation in liver of rats Willumsen,N.;Hexeberg,S.;Skorve,J.;Lundquist,M.;Berge,R.K.
  11. Lipids v.26 The comparative effects of dietary α-linolenic acid and fish oil on 4-and 5-series leukotriene formation in vivo Whelan,J.;Broughton,K.S.;Kinsella,J.E.
  12. J Biol Chem v.253 The site of inhibition of fatty acid oxidation by malonyl CoA McGarry,J.D.;Leatherman,G.F.;Foster,D.W.
  13. Anal Biochem v.50 A rapid spectrophotometric assay for carnitine palmitoyltransferase Bieber,L.L.;Abraham,T.;Helmrath,T.
  14. Methods Enzymol v.72 Protein determinations in membrane and lipoprotein samples : Manual and automated procedures Markwell,M.A.K.;Haas,S.M.;Tolbert,N.E.;Bieber,L.L.
  15. Clin Chim Acta v.37 A method for the determination of carnitine in the picomole range Cederblad,G.;Lindstedt,S.
  16. Am J Clin Nutr v.39 Ameliorating effects of carnitine and its precursors on alcohol-induced fatty liver Sachan,D.S.;Rhew,T.H.;Ruark,R.
  17. J Nutr v.120 Dietary polyunsaturated fats uniquely suppress rat liver fatty acid synthase and S14 mRNA content Clark,S.D.;Aremstrong,M.K.;Jump,D.J.
  18. J Nutr v.120 Suppression of hepatic fatty acid cynthase and S14 gene transcription by dietary polyunsaturated fat Blake,W.C.;Clarke,S.D.
  19. Metabolism v.34 Reduced triglyceride formation from long-chain polyenoic fatty acids in rat hepatocytes Wong,S.;Reardon,M.;Nestel,P.
  20. Lipids v.27 Effect of dietary fish oil on the rate of very low density lipoprotein triacyglycerol formation and on the metabolism of chylomicrons Lottenberg,A.H.;Oliveira,H.C.F.;Nakandakare,E.R.;Quintao,E.C.R.
  21. J Lipid Res v.37 no.3 Stimulation of the activities of hepatic fatty acid oxidation enzymes by dietary fat rich in alpha-linolenic acid in rats Ide,T.;Murata,M.;Sugano,M.
  22. Biochim Biophys Acta v.1085 Metabolic aspects of peroxisomal β-oxidation Osmundsen,H.;Bremer,J.;Pedersen,J.I.
  23. Am J Physiol v.238 Carnitine metabolism in normal weight and obese human subjects during fasting Hoppel,C.L.;Genuth,S.M.
  24. J Biol Chem v.253 Carnitine metabolism in fasting rat Brass,E.P.;Hoppel,C.S.
  25. Biochem J v.249 Enzymes of carnitine acylation Healy,M.J.;Kerner,J.;Bieber,L.L.
  26. Biochem J v.260 Co-ordinate induction of hepatic mitochondrial and peroxisomal carmitine acyl transferase synthesis by and drugs Brady,P.S.;Marine,M.A.;Brady,L.J.;Ramsay,R.R.
  27. J Biol Chem v.261 β-oxidation of polyunsaturated fatty acids by rat liver peroxisomes Hiltunen,J.K.;Karki,T.;Hassinen,I.E.;Osmundsen,H.
  28. Arch Biochem Biophys v.259 Very long chain fatty acid β-oxidation by rat liver mitochondria and peroxisomes Singh,H.;Derwas,N.;Poulos,A.