Isolation and Identification of Carbon Monxide Utilzing Anaerobe, Eubacterium limosum KIST612

일산화탄소를 이용하는 혐기성 세균 Eubacterium limosum KIST612의 분리 및 동정

  • 장인섭 (한국과학기술연구원 환경연구센터) ;
  • 김도희 (한국과학기술연구원 환경연구센터) ;
  • 김병홍 (한국과학기술연구원 환경연구센터) ;
  • 신평균 (한국과학기술연구원 환경연구센터) ;
  • 윤정훈 (한국과학기술연구원 생명공학연구소 유전자원센터 유전자은행 생물정보) ;
  • 이정숙 (한국과학기술연구원 생명공학연구소 유전자원센터 유전자은행 생물정보) ;
  • 박용하 (한국과학기술연구원 생명공학연구소 유전자원센터 유전자은행 생물정보)
  • Published : 1997.02.01

Abstract

Carbon monoxide (CO)-utilizing acetogens were enriched and KIST612 isolated from anaerobic digester fluid was selected for its abilities to tolerate high CO and acetate concentration. The isolate KIST612 was identified as Eubacterium limosum based on the morphological and biochemical characteristics, G+C content of DNA and 16S rRNA sequence analysis. E. limosum KIST612 produced acetate and butyrate from CO. The optimum temperature and pH for the growth and acids formations were 37$\cdot $C and 7.0, respectively. The growth rate and acids productivity of E. limosum KIST612 were higher than those of any other known acetogens when CO was used as the sole energy and carbon source.

Keywords

References

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