Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Production of Lipocortin-1$_{1-185}$ Using A Recombinant of Escherichia coli.

  • Lee, Kyung-Il (Research Center for Molecular Microbiology, Seoul National University) ;
  • Oh, Kyung-Hee (Research Center for Molecular Microbiology, Seoul National University) ;
  • Lee, Jung-Hyun (Research Center for Molecular Microbiology, Seoul National University) ;
  • Na, Do-Sun (Ulsan University) ;
  • Lee, Kye-Joon (Research Center for Molecular Microbiology, Seoul National University)
  • Published : 1997.06.01
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Abstract

The aim of the present study was to optimize culture condition for the expression of lipocortin-1$_{1-185}$ in a recombinant of Escherichia coli using batch system. Plasmid (pHT22) carrying lipocortin-1$_{1-185}$ gene was well maintained in the recombinant with the addition of amplicillin as a selection pressures. Optimum temperature was 28$^{\circ}C$ for seed culture and 4$0^{\circ}C$ for main culture and the optimum pH was 7.0. The production of Lipocortin-1$_{1-185}$ was closely associated with cell growth and related to plasmid amplification.

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