• Title/Summary/Keyword: Lipocortin-1$_{1-185}$ E.coli

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Production of Lipocortin-1$_{1-185}$ Using A Recombinant of Escherichia coli.

  • Lee, Kyung-Il;Oh, Kyung-Hee;Lee, Jung-Hyun;Na, Do-Sun;Lee, Kye-Joon
    • Journal of Microbiology
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    • v.35 no.2
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    • pp.123-126
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    • 1997
  • The aim of the present study was to optimize culture condition for the expression of lipocortin-1$_{1-185}$ in a recombinant of Escherichia coli using batch system. Plasmid (pHT22) carrying lipocortin-1$_{1-185}$ gene was well maintained in the recombinant with the addition of amplicillin as a selection pressures. Optimum temperature was 28$^{\circ}C$ for seed culture and 4$0^{\circ}C$ for main culture and the optimum pH was 7.0. The production of Lipocortin-1$_{1-185}$ was closely associated with cell growth and related to plasmid amplification.

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