Formation of humus-bound residues in the course of BTX biodegradation in soil

  • 발행 : 1997.03.01

초록

To examine whether the xylene component of BTX (benzene, toluene, xylene) mixture is cometabolized and residues are produced in soil, $\^$14/C-labeled-0-xylene was added to sandy loam in combination with unlabeled benzene and toluene. After 4 weeks of incubation in a sealed system connected to an oxygen reservoir, 55.1% of the radiocarbon was converted to $\^$14/CO$\sub$2/, 3.0% was to 95.8% radiocarbon recovery. Biomass incorporation of o-xylene radiocarbon which was detected by fumigation/extraction was usually low (5.6%), but 32.1% radiocarbon became associated with soil humus. Most of the numus-bound radiocarbon was found in humin fraction. In addition to o-xylene, p-xylene and toluene also showed similar results. The evidence shows that some of their reactive methylcatechol biodegradation intermediates attach to the humic metrix in soil in preference to mineralization and biomass incorporation.

키워드

참고문헌

  1. Methods of soil analysis, Amer. Soc. Agron. Total carbon Allison, L.E.;W.B. Bollen;C.D. Moodie;C.A Black(et al.)(eds.)
  2. Appl. Environ. Microbiol. v.57 Substrate interactions of benzene, toluene, and para-xylene during microbial degradation by pure cultures and mixed culture aquifer slurries Alvarez, P.;T. Vogel
  3. Appl. Environ. Microbiol. v.55 Substrate interactions during aerobic biodegradfation of benzene Arvin, E.;B. Jensen;A. Gundersen
  4. Microbiol. Rev. v.45 Microbial degradation of petroleum hydrocarbons: an environmental perspective Atlas, R.M.
  5. Microbiol Ecology Atlas, R.;R. Bartha
  6. Appl. Microbiol. v.18 Rapid technique for enumeration and isolation of peroxidase-producing microorganisms Bartha, R.;L. Bordeleau
  7. Biotechnol. Bioeng. v.37 The biodegradation of mixtures of organic solvents by mixed and monocultures of bacteria Bitzi, U.;T. Egli;G. Hammer
  8. Environ. Sci. Technol. v.26 Decontaminating soil with enzymes Bollag, J.
  9. Biotechnol. Bioeng. v.41 Kinetics of competitive inhibition and cometabolism in the biodegradation of benzene, toluene, and p=xylene by two Pseudomonas isolates Chang, M.;T. Voice;C. Criddle
  10. Soil Biol. Biochem. v.25 Measurement of microbial biomass inarctic tundra soils using fumigation extraction and substrate-induced respiration procedures Cheng, W.;R. Virginia
  11. Microbial degradation of organic compounds Microbial degradation of aromatic hydrocarbons Gibson, D.;V. Subramanian;D. Gibson(ed.)
  12. Appl. Environ. Microbiol. v.58 Biodegradation of mixtures of substituted benzenes by Pseudomonas sp. strain JS150 Haigler, B.;C. Pettigrew;J. Spain
  13. Soil Science v.116 Interaction of pesticide derived chloroaniline residues with soil organic matter Hsu, T.;R. Bartha
  14. Microbiol. Rev. v.54 Microbial degradation of hydrocarbons in the environment Leahy, J.;R. Colwell
  15. Biotechnol. Bioeng. v.43 Metabolic engineering of Pseudonomas putida for the simultaneous biodegradation of benzene, toluene, and p-xylene mixture Lee, J.;J. Roh;H. Kim
  16. Biotechnol. Bioeng. v.33 Dual substrate removal by an axenic bacterial culture Machado, R.;C. Grady
  17. Appl. Environ. Microbiol. v.38 Apparatus for monitoring the mineralization of volatile $^{14}C-labeled compounds Marinucci, A.C.;R. Bartha
  18. Environ. Toxicol. Chem. v.3 Inhibitory interactions of aromatic organics during microbial degradation Meyer, J.;M. Marcus;H. Bergman
  19. Biotechnol. Bioeng. v.44 Interactions between benzene, toluene and p-xyene (BTX) during their biodegradation Oh, Y.-S;Z. Shareefdeen;B.C. Baltzis;R. Bartha
  20. J. Bacteriol. v.124 Metabolism of toluene and xylene by Pseudomonas putida (arvilla) mt-2: evidence for a new function of the TOL plasmid Worsey, M.J.;P.A. Williams
  21. Bull. Environ. Contam. Toxicol. v.29 Evaluation of a chemically defined model for the attachment of 3,4-dichloroaniline to humus You, I.;R. Jones;R. Bartha