Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Toxic Effect of Inhaled Toluene on the Neural Cell

톨루엔 흡입이 신경세포에 미치는 독성

  • 김대병 (식품의약품안전본부 독성연구소) ;
  • 류종훈 (식품의약품안전본부 독성연구소) ;
  • 신대섭 (식품의약품안전본부 독성연구소) ;
  • 이종권 (식품의약품안전본부 독성연구소) ;
  • 정경자 (식품의약품안전본부 독성연구소) ;
  • 류승렬 (식품의약품안전본부 독성연구소) ;
  • 최기환 (식품의약품안전본부 독성연구소) ;
  • 이선희 (식품의약품안전본부 독성연구소) ;
  • 김부영 (식품의약품안전본부 독성연구소)
  • Published : 1997.09.01
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Abstract

Toluene inhalation increases glutamate level and its receptor in various brain regions. In this study, nitric oxide synthase (NOS) activities were investigated in various rat brain regions using NADPH diaphorase staining method which examined histochemical changes of NOS in the neural cells. Also, in vitro LDH leakage assay and MTT test were performed to investigate the toxic influences of toluene in cultured granule cell of rat cerebellum which was significantly affected with toluene in vivo. Rats were exposed to toluene of 10000 ppm for 3 days. 7 days and 14 days by 20 min $\times$ 2 times a day. NADPH diaphorase staining was processed in the different brain regions after inhalation. NADPH diaphorase staining density was not significantly changed at 3 days inhalation group, but the density decreased in proportion to the duration of toluene inhalation. Over 30% of staining density was decreased at 14 days group which was maximum duration of inhalation in this study. The tendency of staining density decrease was significant in granule cell of cerebellum. Cell death by toluene exposure was observed in cultured cerebellar granule cell. $EC_{50}$ measured with LDH leakage assay and MTT test were 43 mM and 72 mM respectively.

Keywords

References

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