Journal of Chest Surgery

The Korean Society for Thoracic and Cardiovascular Surgery (대한심장혈관흉부외과학회)
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Effects of Verapamil in Cardioplegic Perfusates on the Ischemic Myocardium in Isolated Rat Heart

흰쥐의 적출된 심장에서 Verapamil이 허혈성 심근에 미치는 효과

  • Kim, Su-Cheol (Dept. of Thoracic and Cardiovascular Surgery, Kyung Hee University) ;
  • Jo, Gyu-Seok (Dept. of Thoracic and Cardiovascular Surgery, Kyung Hee University) ;
  • Park, Ju-Cheol (Dept. of Thoracic and Cardiovascular Surgery, Kyung Hee University) ;
  • Yu, Se-Yeong (Dept. of Thoracic and Cardiovascular Surgery, Kyung Hee University)
  • 김수철 (경희대학교 의과대학 흉부외과학교실) ;
  • 조규석 (경희대학교 의과대학 흉부외과학교실) ;
  • 박주철 (경희대학교 의과대학 흉부외과학교실) ;
  • 유세영 (경희대학교 의과대학 흉부외과학교실)
  • Published : 1997.02.01
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Abstract

Using isolated rat heart preparations, we observed the protective effe ts of verapamil cardioplegia on ischemic myocardial injury. Isolated rat hearts were subjected to global ischemia at $25^{\circ}C$ Twenty four isolated Sprague Dawley rat hearts underwent 30 minutes of the retrograde nonworking perfusion with Krebs-Henseleit buffer solution followed by $25^{\circ}C$ cardioplegic solution (St. Thomas'Hospital Cardioplegic Solution) for 60 minutes. Before ischemic arrest, rat hearts were treated with cold cardioplegic solution in control group (n=12) and cold cardioplegic solution with verapamil (1 mg/L) in experimental group (n=12). After 60 minutes of ischemia, hemodynamic and biochemical parameters such as heart rate, left ventricular pressure (LVP), + dp/dt max, coronary flow and creatine phosphokinase (CPK) were measured before giving cardioplegia and 30 minutes after reperfusion. Verapamil group exhibited greater recovery of heart rate, LVP, +dpldt max, coronary flow and CPK than control group (p < 0.05).

저자들은 흰쥐의 적출된 심장을 이용하여 Verapamil이 허혈성 심근에 대하여 심근보호효과가 있는지를 관찰하였다 흰쥐의 적출된 심장은 허혈상태에서 심근온도가 $25^{\circ}C$가 되게 유지하였다. 24마리의 흰쥐로부터 적출된 심장을 Krebs-Henseleit완충액을 사용하여 30분간의 비작업성 역관류로 안정시킨 후 $25^{\circ}C$의 심정지액 (St. Thomas' Hospital Cardioplegic Solution)에 60분 동안 저장하였다. 허혈성 심정지를 유도하기 전에 적출된 심장을 저온의 심정지액으로 처리한 군을 대조군(n=12)으로 하고, Verapamil 이 첨가된 저온의 심정지액으로 처리한 군을 실험군(n=12)으로 하였다. 60분 동안의 허혈성 심정지후 심정지전에 측정했던 혈역학적 및 생화학적 지표인 심박동수, 좌심실압, +dpfdt max, 관상관류량과 CPK치를 재관류후 30분에 재측정하여 심정지전과 비교하여 심기능 회복 정도를 관찰하였다. Verapamil이 첨가된 저온의 심정지액을 사용한 실험군이 심박동수, 좌심실압, +dp/dt max, 관상관류량과 CPK치에서 대조군에 비하여 유의하게 높은 회복율을 나타내었다(p <0.05).

Keywords

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