Korean Journal of Animal Reproduction (한국가축번식학회지)
- Volume 21 Issue 1
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- Pages.71-78
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- 1997
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- 1226-5284(pISSN)
Parthenogenetic Activation and Development of Freshly Matured Bovine IVM Oocytes
체외성숙 직후 소 난포란의 단위발생과 체외발육능
Abstract
This study was conducted to investigate the activation condition of freshly matured bovine IVM oocytes for use as a cytoplasmic recipient in nuclear transfer. Bovine oocytes matured in vitro for 22-24 h were treated with various activation conditions. In Experiment 1 in vitro matured oocytes were treated with electric stimuIus (ES; 2 pulses of 1.25 kV/cm for 70
본 연구는 체외성숙 직후의 소 미수정란의 활성화 조건을 검토하기 위하여 실시하였다. 체외에서 22~24시간 성숙배양된 소 미수정란을 다양한 활성화 조건으로 처리하였다. 실험 1에서는 성숙직후 난자를 전기자극(1.25kV/cm, 70