Archives of Pharmacal Research

  • 제20권5호
  • /
  • Pages.486-490
  • /
  • 1997
  • /
  • 0253-6269(pISSN)
  • /
  • 1976-3786(eISSN)
대한약학회 (The Pharmaceutical Society of Korea)
권호 표지

Resolution of Salbutamol Enantiomers in Human Urine by Reversed-phase High Performance Liquid Chromatography after Derivatization with (S)-(-)-${\alpha}$-methylbenzyl isocyanate

  • Kim, Kyeong-Ho (College of Pharmacy, Kangwon National University) ;
  • Kim, Tae-Kyun (Institute of Life Science Research, Choseon Pharm and Trading Co.) ;
  • Kwon, Young-Hee (Department of Pharmacy, Seoul National University Hospital) ;
  • Sohn, Young-Teak (College of Pharmacy, Ducksung Womens University)
  • 발행 : 1997.10.01
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초록

A stereospecific HPLC method has been developed for the resolution of the enantiomers of salbutamol in human urine. After solid-phase extraction and derivatization with (S)-(-)-${\alpha}$-methyl-benzyl isocyanate, the diastereomeric derivatives were resolved $(R_s=1.59)$ on $5{\mu}M$ octadecylsilan column using 47% methanol as a mobile phase with fluorescence detection. The detection limit of each enantiomer was 10 ng/ml (S/N=3).

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참고문헌

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