Archives of Pharmacal Research

The Pharmaceutical Society of Korea (대한약학회)
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Resolution of Salbutamol Enantiomers in Human Urine by Reversed-phase High Performance Liquid Chromatography after Derivatization with (S)-(-)-${\alpha}$-methylbenzyl isocyanate

  • Kim, Kyeong-Ho (College of Pharmacy, Kangwon National University) ;
  • Kim, Tae-Kyun (Institute of Life Science Research, Choseon Pharm and Trading Co.) ;
  • Kwon, Young-Hee (Department of Pharmacy, Seoul National University Hospital) ;
  • Sohn, Young-Teak (College of Pharmacy, Ducksung Womens University)
  • Published : 1997.10.01
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Abstract

A stereospecific HPLC method has been developed for the resolution of the enantiomers of salbutamol in human urine. After solid-phase extraction and derivatization with (S)-(-)-${\alpha}$-methyl-benzyl isocyanate, the diastereomeric derivatives were resolved $(R_s=1.59)$ on $5{\mu}M$ octadecylsilan column using 47% methanol as a mobile phase with fluorescence detection. The detection limit of each enantiomer was 10 ng/ml (S/N=3).

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References

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