Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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PCR-mediated RFLP to Identify 'Bangpoong', a Crude Drug

유전자 감식에 의한 방풍(防風)의 감별

  • Choi, Ho-Young (College of Oriental Medicine, Sangji University) ;
  • Lee, Sang-In (College of Oriental Medicine, Kyung Hee University) ;
  • Suh, Young-Bae (Natural Products Resarch Institute, Seoul National University)
  • 최호영 (상지대학교 한의과대학) ;
  • 이상인 (경희대학교 한의과대학) ;
  • 서영배 (서울대학교 천연물과학연구소)
  • Published : 1997.03.30
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Abstract

Bangpoong (防風) is a popular crude drug used to expel wind from the body surface (祛風解表) to remove dampness (勝濕). And to relieve pain (正痛) and spasm (正痙). In China and Japan, roots of Saposhnikovia divaricata (Turcz.) Schischk. Is used as Bangpoong. However, the roots of Peucedanum japonicum Thunb. Or Glehnia littoralis (A. Gray) Fr. Schmidt ex Miquel, being called Sikbangpoong (植防風) and Wonbangpoong (元防風) respectively are used instead of Bangpoong in Korea. The ITS regions of nuclear ribosomal DNA were analyzed to determine original plants and to design a molecular identification method for the crude drugs used as Bangpoong in Korea and China. It is demonstrated that RFLP analysis via PCR has the great potential as a novel tool to test crude drugs for the quality control and standardization.

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